SWAP-70 promotes glioblastoma cellular migration and invasion by regulating the expression of CD44s

Cancer Cell International

Jun Liang¹  Xu Zhang¹  Lin Shi²  Yifeng Wang²  Xiangyu Chen²  Xuejiao Liu²  Rutong Yu²  Huan Li²  Yulong Chong²  Jie Wang²  Guanzheng Liu²  Mingshan Niu²  Huize Liu² 

[1] Department of Neurosurgery, Affiliated Hospital of Xuzhou Medical University;Institute of Nervous System Diseases, Affiliated Hospital of Xuzhou Medical University, Xuzhou Medical University;

关键词: Glioblastoma;    SWAP-70;    CD44s;    Migration;    Invasion;   

DOI  :  10.1186/s12935-019-1035-3

来源: DOAJ

【 摘 要 】

Abstract Background Switch-associated protein 70 (SWAP-70) is a guanine nucleotide exchange factor that is involved in cytoskeletal rearrangement and regulation of migration and invasion of malignant tumors. However, the mechanism by which SWAP-70 regulates the migration and invasion of glioblastoma (GB) cells has not been fully elucidated. Methods This study used an online database to analyze the relationship between SWAP-70 expression and prognosis in GB patients. The in vitro wound healing assay and transwell invasion assay were used to determine the role of SWAP-70 in GB cell migration and invasion as well as the underlying mechanism. Results We found that patients with high SWAP-70 expression in the GB had a poor prognosis. Downregulation of SWAP-70 inhibited GB cell migration and invasion, whereas SWAP-70 overexpression had an opposite effect. Interestingly, SWAP-70 expression was positively correlated with the expression of the standard form of CD44 (CD44s) in GB tissues. Downregulation of SWAP-70 also reduced CD44s protein expression, whereas SWAP-70 overexpression enhanced CD44s protein expression. However, downregulation of SWAP-70 expression did not affect the mRNA expression of CD44s. Reversal experiments showed that overexpressing CD44s in cell lines with downregulated SWAP-70 partially abolished the inhibitory effects of downregulated SWAP-70 on GB cell migration and invasion. Conclusions These results suggest that SWAP-70 may promote GB cell migration and invasion by regulating the expression of CD44s. SWAP-70 may serve as a new biomarker and a potential therapeutic target for GB.

【 授权许可】

Unknown