Characterization of Nivalenol-Producing Fusarium culmorum Isolates Obtained from the Air at a Rice Paddy Field in Korea

The Plant Pathology Journal

Jueun Kim¹  Chul Won Lee¹  Hee-Kyoung Kim²  Da-Woon Kim²  Gi-Yong Kim²  Sung-Hwan Yun²  Sun Jeong Jeon³  Hyang Burm Lee³ 

[1] Department of Chemistry, Chonnam National University, Gwangju 61186, Korea;Department of Medical Biotechnology, Soonchunhyang University, Asan 31538, Korea;Division of Food Technology, Biotechnology & Agrochemistry, Chonnam National University, Gwangju 61186, Korea;

关键词: Fusarium culmorum;    Fusarium head blight;    nivalenol production;    rice paddy field air;   

DOI  :  10.5423/PPJ.OA.12.2015.0268

来源: DOAJ

【 摘 要 】

Together with the Fusarium graminearum species complex, F. culmorum is a major member of the causal agents of Fusarium head blight on cereals such as wheat, barley and corn. It causes significant yield and quality losses and results in the contamination of grain with mycotoxins that are harmful to humans and animals. In Korea, F. culmorum is listed as a quarantine fungal species since it has yet to be found in the country. In this paper, we report that two isolates (J1 and J2) of F. culmorum were collected from the air at a rice paddy field in Korea. Species identification was confirmed by phylogenetic analysis using multi-locus sequence data derived from five genes encoding translation elongation factor, histone H3, phosphate permease, a reductase, and an ammonia ligase and by morphological comparison with reference strains. Both diagnostic PCR and chemical analysis confirmed that these F. culmorum isolates had the capacity to produce nivalenol, the trichothecene mycotoxin, in rice substrate. In addition, both isolates were pathogenic on wheat heads and corn stalks. This is the first report on the occurrence of F. culmorum in Korea.

【 授权许可】

Unknown