Influence of the Incubator as Direct Patient Environment on Bacterial Colonization of Neonates

Microorganisms

Kristin Dawczynski¹  Frank Kipp²  Isabel Lange²  Mathias W. Pletz²  Claudia Stein²  Birgit Edel³  Hans Proquitté⁴ 

[1] Department of Pediatrics Weimar, Sophien-und Hufeland Klinikum, Henry-van-de-Velde-Str. 1, 99425 Weimar, Germany;Institute for Infectious Diseases and Infection Control, Jena University Hospital, Am Klinikum 1, 07747 Jena, Germany;Institute of Medical Microbiology, Jena University Hospital, Am Klinikum 1, 07747 Jena, Germany;Unit of Neonatology, Department of Paediatrics, Jena University Hospital, Friedrich Schiller University Jena, 07747 Jena, Germany;

关键词: healthcare-associated infections;    colonization;    neonatal care;    incubator;    whole genome sequencing;    infection control;   

DOI  :  10.3390/microorganisms9122533

来源: DOAJ

【 摘 要 】

Background: Preventing healthcare-associated infections (HAI) in neonatal intensive care units is a challenge of highest priority. For further insight into the incubator as direct patient environment and potential source for contamination, we present data correlating microbiological samples of very low birthweight infants in the form of colonization results of surveillance screenings with samples of their associated incubator in this study. Methods: Samples were taken via rectal and throat swabs of neonates as well as Polywipe^® sponges for the incubator. If the same bacterial species was found in corresponding neonate and incubator samples, whole genome sequencing via Illumina technology was performed. Results: 52 microbiological species matches were found, and 30 matches were sequenced where we found 26 clonal pairs (12 E. faecalis, 10 S. aureus, 2 E. coli, 1 E. cloacae, and 1 E. faecium). Conclusion: The combinations of measurements of weekly screenings swabs, probing of surfaces with Polywipes^®, and whole genome sequencing showed transmissions of microorganism and risk for potential non-physiological colonization of neonatal infants.

【 授权许可】

Unknown