The rapid survey method of chemical contamination in floods caused by Typhoon Hagibis by combining in vitro bioassay and comprehensive analysis

Environment International

Yuichi Miyabara¹  Kiwao Kadokami²  Masashi Toyota³  Shunji Hashimoto⁴  Ryo Omagari⁵  Takashi Miyawaki⁵  Daisuke Nakajima⁶ 

[1] Corresponding author at: Center for Health and Environmental Risk Research, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba-shi, Ibaraki-ken 305-8506, Japan.;Faculty of Engineering, Water Environment and Civil Engineering, Shinshu University, Japan;Faculty of Environmental Engineering, The University of Kitakyushu, Japan;Faculty of Science, Suwa Hydrobiological Station, Shinshu University, Japan;Health and Environmental Risk Division, National Institute for Environmental Studies, Japan;Institute of Environmental Science and Technology, The University of Kitakyushu, Japan;

关键词: AIQS-GC;    Yeast two-hybrid assay;    Typhoon Hagibis;    Flood event;    DDT;    Human health risk;   

DOI  :  

来源: DOAJ

【 摘 要 】

A novel comprehensive assessment system, consisting of a bioassay and chemical analysis, was developed to quickly evaluate the human health risk posed by toxic chemicals discharged due to natural disasters. To analyze samples quickly, a yeast-two-hybrid assay (Y2H) and GC–MS equipped with an automated identification and quantification system (AIQS-GC) were employed for the bioassay and chemical analysis, respectively. Since the analysis of 1000 substances by AIQS could be finished within two days following the Y2H assay for screening, this method would complete the risk assessment within three days. To confirm the applicability of this method in real environmental samples, we examined it using sediments circulated by Typhoon Hagibis. In one sediment sample, a distinctive response was indicated by the Y2H assay, and relatively high DDT concentration was identified by AIQS-GC in the same sediment. Therefore, using the results obtained from this method, a human health risk assessment of DDT was conducted, which indicated that the risk could be ignored. Additionally, the contamination of PAHs and alkanes was suggested as well. In this study, the pollution risk assessment could be completed within three days. Therefore, to our knowledge, this is the first study to demonstrate an assessment system with a rapid combination method for emergencies. Consequently, it is believed that this type of novel system would be needed in the future due to the increasing number of natural disasters predicted worldwide.

【 授权许可】

Unknown