Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversion

Biotechnology Reports

Mercedes M. Garrido¹  Ruben Marrero Diaz de Villegas²  Eleonora Campos²  Silvina Ghio²  María B. Bradanini²  Florencia E. Piccinni²  Ornella M. Ontañon²  Paola M. Talia² 

[1] University of San Martin (UNSAM), Buenos Aires, Argentina;Institute for Agrobiotechnology and Molecular Biology (IABIMO), National Institute for Agricultural Technology (INTA) and National Council for Scientific and Technological Research (CONICET), Argentina;

关键词: Endoglucanse;    GH8;    Beta-glucosidase;    GH1;    Paenibacillus;    Beta-glucan;   

DOI  :  

来源: DOAJ

【 摘 要 】

In the efficient bioconversion of polysaccharides from lignocellulosic biomass, endoglucanases and β-glucosidases are key enzymes for the deconstruction of β-glucans. In this work, we focused on a GH8 endoglucanase (Cel8Pa) and a GH1 β-glucosidase (Bg1Pa) from Paenibacillus xylanivorans A59. Cel8Pa was active on a broad range of substrates, such as β-glucan from barley (24.5 IU/mg), lichenan (17.9 IU/mg), phosphoric acid swollen cellulose (PASC) (9.7 IU/mg), carboxi-methylcellulose (CMC) (7.3 IU/mg), chitosan (1.4 IU/mg) and xylan (0.4 IU/mg). Bg1Pa was active on cellobiose (C2) and cello-oligosaccharides up to C6, releasing glucose as the main product. When both enzymes were used jointly, there was a synergic effect in the conversion rate of polysaccharides to glucose. Cel8Pa and Bg1Pa presented important properties for simultaneous saccharification and fermentation (SSF) processes in second generation bioethanol production, such as tolerance to high concentration of glucose and ethanol.

【 授权许可】

Unknown