Preparation and Application of Molecularly Imprinted Monolithic Extraction Column for the Selective Microextraction of Multiple Macrolide Antibiotics from Animal Muscles

Polymers

Yijuan Su¹  Xuqin Song²  Hao Zhou²  Limin He²  Jiahui Zhang²  Tong Zhou² 

[1] Department of Ecology, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China;National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;

关键词: molecularly imprinted monolithic extraction column;    microextraction;    macrolide antibiotics;    animal muscles;    liquid chromatography-tandem mass spectrometry;   

DOI  :  10.3390/polym11071109

来源: DOAJ

【 摘 要 】

This study aimed to prepare a molecularly imprinted monolithic extraction column (MIMC) inside a micropipette tip by situ polymerization with roxithromycin as the dummy template. The polymers possessed excellent adsorption capacity and class-specificity to multiple macrolide drugs. MIMC was directly connected to a syringe for template removal and for the optimization of extraction conditions without any other post-treatment of polymers. A liquid chromatography-tandem mass spectrometric method was developed for the selective microextraction and determination of macrolide antibiotics in animal muscles based on MIMC. High recoveries of 76.1−92.8% for six macrolides were obtained with relative standard deviations less than 10.4%. MIMC exhibited better retention ability and durability when compared with the traditional C18 and HLB cartridges. The proposed method shows a great potential for the analysis of macrolide drugs at the trace level in animal foodstuffs.

【 授权许可】

Unknown