Strategies and Considerations for Improving Recombinant Antibody Production and Quality in Chinese Hamster Ovary Cells

Frontiers in Bioengineering and Biotechnology

Jing-Jing Li¹  Lin-Lin Shan¹  Jun-He Zhang²  Chen-Yang Du³  Fan Liang³ 

[1] Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, China;Henan International Joint Laboratory of Recombinant Pharmaceutical Protein Expression System, Xinxiang Medical University, Xinxiang, China;Institutes of Health Central Plains, Xinxiang Medical University, Xinxiang, China;

关键词: recombinant antibody;    Chinese hamster ovary cells;    expression vector;    glycosylation;    genetic engineering;   

DOI  :  10.3389/fbioe.2022.856049

来源: DOAJ

【 摘 要 】

Recombinant antibodies are rapidly developing therapeutic agents; approximately 40 novel antibody molecules enter clinical trials each year, most of which are produced from Chinese hamster ovary (CHO) cells. However, one of the major bottlenecks restricting the development of antibody drugs is how to perform high-level expression and production of recombinant antibodies. The high-efficiency expression and quality of recombinant antibodies in CHO cells is determined by multiple factors. This review provides a comprehensive overview of several state-of-the-art approaches, such as optimization of gene sequence of antibody, construction and optimization of high-efficiency expression vector, using antibody expression system, transformation of host cell lines, and glycosylation modification. Finally, the authors discuss the potential of large-scale production of recombinant antibodies and development of culture processes for biopharmaceutical manufacturing in the future.

【 授权许可】

Unknown