Stilbene compounds are specific inhibitors of the superoxide anion generation catalyzed by xanthine oxidase

Food Chemistry: X

Noriyoshi Masuoka¹ 

[1] Tsudaka-Fruit Juice Laboratory, Okayama Research-Park Incubation Center, 5303 Haga, Kita-ku, Okayama 701-1221, Japan;

关键词: Flavin adenine dinucleotide;    π-π interaction;    Resveratrol;    Piceatannol;    Superoxide anion inhibitor;    Superoxide anion scavenger;   

DOI  :  

来源: DOAJ

【 摘 要 】

The inhibitory effect of xanthine oxidase (XO) reactions with stilbene compounds, 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging by stilbene compounds and superoxide anion (O2-) scavenging activity were examined. The inhibition of the O2- generation catalyzed by XO by stilbene compounds is stronger than the effect on uric acid formation. The suppression of the O2- generation with resveratrol was diminished by the addition of flavin adenine dinucleotide (FAD). The water-solubility and visible spectra (VIS) of the stilbene compounds in the presence of water-soluble flavin compounds indicated a π-π interaction between the stilbene compounds and the isoalloxazine in flavin compounds. These results indicate that stilbene compounds specifically bind the FAD site in XO so as to inhibit the O2- generation. In the case of piceatannol, it is deduced that the suppression of O2- generation is induced by this specific binding to the FAD site and the subsequent reduction of XO.

【 授权许可】

Unknown