Liquid Chromatograph-Mass Spectrometry-Based Non-targeted Metabolomics Discovery of Potential Endogenous Biomarkers Associated With Prostatitis Rats to Reveal the Effects of Magnoflorine

Frontiers in Pharmacology

Hong-Bin Xiao¹  Yin Yuan²  Xu Liu²  Fei-Xue Dong³  Zhong-Guang Zhou⁴ 

[1] Department of Basic Medicine, College of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin, China;Department of Basic Medicine, Heilongjiang University of Chinese Medicine, Harbin, China;First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, China;Research Institute of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin, China;

关键词: metabolomics;    UPLC-MS;    metabolites;    pathways;    metabolite biomarkers;   

DOI  :  10.3389/fphar.2021.741378

来源: DOAJ

【 摘 要 】

Magnoflorine (Mag) has multiple pharmacological activities for the prevention and treatment of prostatitis. However, its molecular mechanisms andpharmacological targets are not clear. In this study, the ultra-performance liquid tandem mass spectrometry-based metabolomics method was used to clarify the intervention of Mag against prostatitis and the biological mechanism. A total of 25 biomarkers associated with the prostatitis model were identified by metabolomics, and a number of metabolic pathways closely related to the model were obtained by MetPA analysis. After given Mag treatment, the results of each indicator were shown that Mag alkaloid could inhibit the development of prostatitis effectively. We found that Mag had regulative effects on potential biomarkers of prostatitis model, which can regulate them to the control group. Our results indicated that alkaloids have an effective intervention therapy for prostatitis, and five types of metabolic pathways closely related to prostatitis model were obtained, including phenylalanine, tyrosine and tryptophan biosynthesis, phenylalanine metabolism, tyrosine metabolism, arginine and proline metabolism, glycine, serine and threonine metabolism, alanine, aspartate and glutamate metabolism. This study has provided the basic experimental data for the development of Mag in the prevention and treatment of prostatitis.

【 授权许可】

Unknown