Microbiome Patterns in Matched Bile, Duodenal, Pancreatic Tumor Tissue, Drainage, and Stool Samples: Association with Preoperative Stenting and Postoperative Pancreatic Fistula Development

Journal of Clinical Medicine

Stefan Wirtz¹  Abbas Agaimy²  Barbara Kneis³  Christian Krautz³  GeorgF. Weber³  Stephan Kersting³  Christian Pilarsky³  Maximilian Brunner³  Robert Grützmann³  MatthiasM. Gittler³  Melanie Langheinrich³  Jonel Trebicka⁴  Robert Schierwagen⁴  Olaf Tyc⁴ 

[1] Department of Internal Medicine 1, University Hospital of Erlangen, 91054 Erlangen, Germany;Department of Pathology, University Hospital of Erlangen, 91054 Erlangen, Germany;Department of Surgery, University Hospital of Erlangen, 91054 Erlangen, Germany;Translational Hepatology, Department of Internal Medicine I, University Clinic Frankfurt, 60590 Frankfurt, Germany;

关键词: microbiome;    pancreatic surgery;    postoperative complications;    pancreatic cancer;    16S rRNA sequencing;   

DOI  :  10.3390/jcm9092785

来源: DOAJ

【 摘 要 】

Postoperative complications after pancreatic surgery are still a significant problem in clinical practice. The aim of this study was to characterize and compare the microbiomes of different body compartments (bile duct, duodenal mucosa, pancreatic tumor lesion, postoperative drainage fluid, and stool samples; preoperative and postoperative) in patients undergoing pancreatic surgery for suspected pancreatic cancer, and their association with relevant clinical factors (stent placement, pancreatic fistula, and gland texture). For this, solid (duodenal mucosa, pancreatic tumor tissue, stool) and liquid (bile, drainage fluid) biopsy samples of 10 patients were analyzed using 16s rRNA gene next-generation sequencing. Our analysis revealed: (i) a distinct microbiome in the different compartments, (ii) markedly higher abundance of Enterococcus in patients undergoing preoperative stent placement in the common bile duct, (iii) significant differences in the beta diversity between patients who developed a postoperative pancreatic fistula (POPF B/C), (iv) patients with POPF B/C were more likely to have bacteria belonging to the genus Enterococcus, and (v) differences in microbiome composition with regard to the pancreatic gland texture. The structure of the microbiome is distinctive in different compartments, and can be associated with the development of a postoperative pancreatic fistula.

【 授权许可】

Unknown