Progression of Watermelon Bud Necrosis Virus Infection in Its Vector, Thrips palmi

Cells

Amalendu Ghosh¹  Bikash Mandal¹  Priti Saini¹  RalfG. Dietzgen² 

[1] Insect vector laboratory, Advanced Centre for Plant Virology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India;Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, QLD, Brisbane 4072, Australia;

关键词: virus–vector relationships;    thrips;    orthotospovirus;    localization;    WBNV;    melon thrips;   

DOI  :  10.3390/cells10020392

来源: DOAJ

【 摘 要 】

Thrips are important pests of agricultural, horticultural, and forest crops worldwide. In addition to direct damages caused by feeding, several thrips species can transmit diverse tospoviruses. The present understanding of thrips–tospovirus relationships is largely based on studies of tomato spotted wilt virus (TSWV) and Western flower thrips (Frankliniella occidentalis). Little is known about other predominant tospoviruses and their thrips vectors. In this study, we report the progression of watermelon bud necrosis virus (WBNV) infection in its vector, melon thrips (Thrips palmi). Virus infection was visualized in different life stages of thrips using WBNV-nucleocapsid protein antibodies detected with FITC-conjugated secondary antibodies. The anterior midgut was the first to be infected with WBNV in the first instar larvae. The midgut of T. palmi was connected to the principal salivary glands (PSG) via ligaments and the tubular salivary glands (TSG). The infection progressed to the PSG primarily through the connecting ligaments during early larval instars. The TSG may also have an ancillary role in disseminating WBNV from the midgut to PSG in older instars of T. palmi. Infection of WBNV was also spread to the Malpighian tubules, hindgut, and posterior portion of the foregut during the adult stage. Maximum virus-specific fluorescence in the anterior midgut and PSG indicated the primary sites for WBNV replication. These findings will help to better understand the thrips–tospovirus molecular relationships and identify novel potential targets for their management. To our knowledge, this is the first report of the WBNV dissemination path in its vector, T. palmi.

【 授权许可】

Unknown