In vivo spectroscopy and NMR metabolite fingerprinting approaches to connect the dynamics of photosynthetic and metabolic phenotypes in resurrection plant Haberlea rhodopensis during desiccation and recovery

Frontiers in Plant Science

Petko eMladenov¹  Dimitar eDjilianov¹  Daniela eMoyankova¹  Richard eBligny²  Giovanni eFinazzi²  Norbert eRolland²  Sabine eBrugiere³  Myriam eFerro³  Anne-Marie eBoisson³  Diana eZasheva⁴  Kalina eAlipieva⁵  Svetlana eSimova⁵  Vasilena eKrasteva⁶  Magdalena eTchorbadjieva⁶  Vasiliy eGoltsev⁶ 

[1] Agrobioinstitute, Agricultural Academy;Institut de Recherches en Technologies et Sciences pour le Vivant, CEA, CNRS, Univ. Grenoble Alpes. , INRA;Institut de Recherches en Technologies et Sciences pour le Vivant, CEA, Univ. Grenoble Alpes., INSERM,;Institute of Biology and Immunology of Reproduction, Bulgarian Academy of Sciences;Institute of Organic Chemistry with Centre of Phytochemistry;Sofia University, Faculty of Biology;

关键词: Metabolism;    Photosynthesis;    phenotype;    Drought stress;    Haberlea rhodopensis;    resurrection plant;   

DOI  :  10.3389/fpls.2015.00564

来源: DOAJ

【 摘 要 】

The resurrection plant Haberlea rhodopensis was used to study dynamics of drought response of photosynthetic machinery parallel with changes in primary metabolism. A relation between leaf water content and photosynthetic performance was established, enabling us to perform a non-destructive evaluation of the plant water status during stress. Spectroscopic analysis of photosynthesis indicated that, at variance with linear electron flow involving photosystem (PS) I and II, cyclic electron flow around PSI remains active till almost full dry state at the expense of the linear electron flow, due to the changed protein organization of photosynthetic apparatus. We suggest that, this activity could have a photoprotective role and prevent a complete drop in adenosine triphosphate (ATP), in the absence of linear electron flow, to fuel specific energy-dependent processes necessary for the survival of the plant, during the late states of desiccation. The NMR fingerprint show significant metabolic changes in several pathways. Due to the declining of linear electron flow accompanied by biosynthetic reactions during desiccation, a reduction of the ATP pool during drought was observed, which was fully and quickly recovered after plants rehydration. We found a decline of valine accompanied by lipid degradation during stress, likely to provide alternative carbon sources for sucrose accumulation at late stages of desiccation. This accumulation, as well as the increased levels of glycerophosphodiesters during drought stress could provide osmoprotection to the cells.

【 授权许可】

Unknown