Ratiometric G-Quadruplex Assay for Robust Lead Detection in Food Samples

Biosensors

Yumei Liu¹  Ruijie Deng¹  Rui Wan¹  Qiang He¹  Hao Yang¹  Zhifeng Zhao¹  Nan Wang¹  Mohammad Rizwan Khan²  Rosa Busquets³ 

[1] College of Biomass Science and Engineering, Healthy Food Evaluation Research Center and Key Laboratory of Food Science and Technology of Ministry of Education of Sichuan Province, Sichuan University, Chengdu 610065, China;Department of Chemistry, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;School of Life Sciences, Pharmacy and Chemistry, Kingston University London, Penrhyn Road, Kingston-upon-Thames, Surrey KT1 2EE, UK;

关键词: G-quadruplex;    nucleic acid probes;    lead pollution;    food safety;    homogeneous detection;   

DOI  :  10.3390/bios11080274

来源: DOAJ

【 摘 要 】

Lead (Pb²⁺) pollution is a serious food safety issue, rapid detection of Pb²⁺ residual in food is vital to guarantee food quality and safety. Here we proposed ratiometric aptamer probes, allowing robust Pb²⁺ supervision in food samples. Pb²⁺ specific aptamer can bolster a transition of G-quadruplex structural response to Pb²⁺; this process can be monitored by N-methyl mesoporphyrin IX (NMM), which is highly specific to G-quadruplex. Particularly, the utilization of G-quadruplex specific dye and terminal-labeled fluorophore allowed to endue ratiometric signal outputs towards Pb²⁺, dramatically increase the robustness for lead detection. The ratiometric G-quadruplex assay allowed a facile and one-pot Pb²⁺ detection at room temperature using a single-stranded DNA aptamer. We demonstrated its feasibility for detecting lead pollution in fresh eggs and tap water samples. The ratiometric G-quadruplex design is expected to be used for on-site Pb²⁺ testing associated with food safety.

【 授权许可】

Unknown