An in vitro stem cell model of human epiblast and yolk sac interaction

eLife

Lygia V Pereira¹  George Hudson²  Charlotte E Handford³  Viviane Souza Rosa⁴  Kirsty ML Mackinlay⁴  Tim Coorens⁴  Sam Behjati⁴  Bailey AT Weatherbee⁴  Marta N Shahbazi⁵  Magdalena Zernicka-Goetz⁶  Ludovic Vallier⁶ 

[1] Centre for Trophoblast Research, University of Cambridge, Cambridge, United Kingdom;MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, United Kingdom;National Laboratory for Embryonic Stem Cells (LaNCE), Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil;Mammalian Embryo and Stem Cell Group, University of Cambridge, Department of Physiology, Development and Neuroscience, Cambridge, United Kingdom;National Laboratory for Embryonic Stem Cells (LaNCE), Department of Genetics and Evolutionary Biology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil;Wellcome Sanger Institute, Cambridge, United Kingdom;

关键词: embryology;    stem cells;    hypoblast;    human development;    pluripotency;    epiblast;   

DOI  :  10.7554/eLife.63930

来源: DOAJ

【 摘 要 】

Human embryogenesis entails complex signalling interactions between embryonic and extra-embryonic cells. However, how extra-embryonic cells direct morphogenesis within the human embryo remains largely unknown due to a lack of relevant stem cell models. Here, we have established conditions to differentiate human pluripotent stem cells (hPSCs) into yolk sac-like cells (YSLCs) that resemble the post-implantation human hypoblast molecularly and functionally. YSLCs induce the expression of pluripotency and anterior ectoderm markers in human embryonic stem cells (hESCs) at the expense of mesoderm and endoderm markers. This activity is mediated by the release of BMP and WNT signalling pathway inhibitors, and, therefore, resembles the functioning of the anterior visceral endoderm signalling centre of the mouse embryo, which establishes the anterior-posterior axis. Our results implicate the yolk sac in epiblast cell fate specification in the human embryo and propose YSLCs as a tool for studying post-implantation human embryo development in vitro.

【 授权许可】

Unknown