International Journal of Molecular Sciences | |
Brassinosteroids Mitigate Cadmium Effects in Arabidopsis Root System without Any Cooperation with Nitric Oxide | |
Camilla Betti1  Giuseppina Falasca2  Dario Bellanima2  Nicoletta Girardi2  Diego Piacentini2  Laura Fattorini2  Federica Della Rovere2  Maria Maddalena Altamura2  | |
[1] Department of Biosciences, University of Milan, 20133 Milan, Italy;Department of Environmental Biology, Sapienza University of Rome, 00185 Rome, Italy; | |
关键词: adventitious roots; auxin localization; brassinazole; CdSO4; epibrassinolide; lateral roots; | |
DOI : 10.3390/ijms23020825 | |
来源: DOAJ |
【 摘 要 】
The heavy metal cadmium (Cd) affects root system development and quiescent center (QC)-definition in Arabidopsis root-apices. The brassinosteroids-(BRs)-mediated tolerance to heavy metals has been reported to occur by a modulation of nitric oxide (NO) and root auxin-localization. However, how BRs counteract Cd-action in different root types is unknown. This research aimed to find correlations between BRs and NO in response to Cd in Arabidopsis’s root system, monitoring their effects on QC-definition and auxin localization in root-apices. To this aim, root system developmental changes induced by low levels of 24-epibrassinolide (eBL) or by the BR-biosynthesis inhibitor brassinazole (Brz), combined or not with CdSO4, and/or with the NO-donor nitroprusside (SNP), were investigated using morpho-anatomical and NO-epifluorescence analyses, and monitoring auxin-localization by the DR5::GUS system. Results show that eBL, alone or combined with Cd, enhances lateral (LR) and adventitious (AR) root formation and counteracts QC-disruption and auxin-delocalization caused by Cd in primary root/LR/AR apices. Exogenous NO enhances LR and AR formation in Cd-presence, without synergism with eBL. The NO-signal is positively affected by eBL, but not in Cd-presence, and BR-biosynthesis inhibition does not change the low NO-signal caused by Cd. Collectively, results show that BRs ameliorate Cd-effects on all root types acting independently from NO.
【 授权许可】
Unknown