Using RNA-Seq to Identify Reference Genes of the Transition from Brown to White Adipose Tissue in Goats

Animals

Li Li¹  Linjie Wang¹  Tao Zhong¹  Hongping Zhang¹  Xingyue Chen¹  Jiaxue Cao¹  Yan Wang¹  Xujia Zhang¹  Siyuan Zhan¹  Jiazhong Guo¹  Tianzeng Song² 

[1] Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China;Institute of Animal Science, Tibet Academy of Agricultural and Animal Husbandry Science, Lhasa 850009, China;

关键词: goat;    brown adipose tissue;    reference gene;    Normfinder;    BestKeeper;    geNorm;   

DOI  :  10.3390/ani10091626

来源: DOAJ

【 摘 要 】

Brown adipose tissues have unique non-shivering thermogenesis functions, can be found in newborn ruminate animals, and then are gradually replaced by white adipose tissues in adulthood. For the purpose of exploring the intrinsic mechanism underlying the conversion process from brown (BAT) to white adipose tissue (WAT), it is necessary to utilize Quantitative PCR (qPCR) to study gene expression profiling. In this study, we identified reference genes that were consistently expressed during the transformation from goat BAT to WAT using RNA-seq data. Then, twelve genes were evaluated as candidate reference genes for qPCR in goat perirenal adipose tissue using three tools (geNorm, Normfinder, and BestKeeper). In addition, the selected reference genes were used to normalize the gene expression of PGC-1α and GPAT4. It was found that traditional reference genes, such as GAPDH, RPLP0, HPRT1, and PPIA were not suitable for target gene normalization. In contrast, CTNNB, PFDN5, and EIF3M, selected from RNA sequencing data, showed the least variation and were recommended as the best reference genes during the transformation from BAT to WAT.

【 授权许可】

Unknown