Response to a DNA vaccine against the H5N1 virus depending on the chicken line and number of doses

Virology Journal

Krzysztof Śmietanka¹  Monika Olszewska-Tomczyk¹  Anna Fogtman²  Róża Sawicka²  Agnieszka Sirko²  Barbara Małgorzata Kalenik²  Anna Stachyra²  Anna Góra-Sochacka² 

[1]Department of Poultry Diseases, National Veterinary Research Institute

[2]Institute of Biochemistry and Biophysics, Polish Academy of Sciences

关键词: Broilers;    Chicken;    DNA vaccine;    Influenza;    Layers;    Spleen transcriptome;   

DOI  :  10.1186/s12985-020-01335-9

来源: DOAJ

【 摘 要 】

Abstract Background Avian influenza virus infections cause significant economic losses on poultry farms and pose the threat of a possible pandemic outbreak. Routine vaccination of poultry against avian influenza is not recommended in Europe, however it has been ordered in some other countries, and more countries are considering use of the avian influenza vaccine as a component of their control strategy. Although a variety of such vaccines have been tested, most research has concentrated on specific antibodies and challenge experiments. Methods We monitored the transcriptomic response to a DNA vaccine encoding hemagglutinin from the highly pathogenic H5N1 avian influenza virus in the spleens of broiler and layer chickens. Moreover, in layer chickens the response to one and two doses of the vaccine was compared. Results All groups of birds immunized with two doses of the vaccine responded at the humoral level by producing specific anti-hemagglutinin antibodies. A response to the vaccine was also detected in the spleen transcriptomes. Differential expression of many genes encoding noncoding RNA and proteins functionally connected to the neuroendocrine-immune system was observed in different immunized groups. Conclusion Broiler chickens showed a higher number and wider range of fold-changes in the transcriptional response than laying hens.

【 授权许可】

Unknown