Highly Promiscuous Oxidases Discovered in the Bovine Rumen Microbiome

Frontiers in Microbiology

Diego P. Morgavi¹  Angeline Rizzo²  Davide Cecchini²  Alexandra S. Tauzin²  Megane Cleret²  Lisa Ufarté²  Gabrielle Potocki-Veronese²  Elisabeth Laville²  Patrick Robe³  Christophe Klopp⁴  Bernard Cathala⁵  Céline Moreau⁵ 

[1] INRA, UMR 1213 Herbivores, Saint-Genès Champanelle, France;Laboratoire d’Ingénierie des Systèmes Biologiques et des Procédés, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Institut National des Sciences Appliquées de Toulouse, Université de Toulouse, Toulouse, France;LibraGen S.A, Toulouse, France;Plateforme Bio-informatique Toulouse Genopole, UBIA INRA, BP 52627, Castanet-Tolosan, France;UR1268 Biopolymères Interactions Assemblages, INRA, Nantes, France;

关键词: bovine rumen;    microbiome;    functional metagenomics;    lignin degradation;    redox enzymes;    biorefining;   

DOI  :  10.3389/fmicb.2018.00861

来源: DOAJ

【 摘 要 】

The bovine rumen hosts a diverse microbiota, which is highly specialized in the degradation of lignocellulose. Ruminal bacteria, in particular, are well equipped to deconstruct plant cell wall polysaccharides. Nevertheless, their potential role in the breakdown of the lignin network has never been investigated. In this study, we used functional metagenomics to identify bacterial redox enzymes acting on polyaromatic compounds. A new methodology was developed to explore the potential of uncultured microbes to degrade lignin derivatives, namely kraft lignin and lignosulfonate. From a fosmid library covering 0.7 Gb of metagenomic DNA, three hit clones were identified, producing enzymes able to oxidize a wide variety of polyaromatic compounds without the need for the addition of copper, manganese, or mediators. These promiscuous redox enzymes could thus be of potential interest both in plant biomass refining and dye remediation. The enzymes were derived from uncultured Clostridia, and belong to complex gene clusters involving proteins of different functional types, including hemicellulases, which likely work in synergy to produce substrate degradation.

【 授权许可】

Unknown