Is the Activity-Based Anorexia Model a Reliable Method of Presenting Peripheral Clinical Features of Anorexia Nervosa?

Nutrients

Beata Kuśnierz-Cabala¹  Paulina Mazur²  Kamil Skowron³  Paulina Stach³  Krzysztof Gil³  Veronika Aleksandrovych³  Magdalena Kurnik-Łucka³  Michał Jurczyk³  Emil Dadański³  Krzysztof Wąsowicz⁴  Piotr Podlasz⁴  Krzysztof Jasiński⁵  Władysław P. Węglarz⁵ 

[1] Department of Diagnostics, Faculty of Medicine, Jagiellonian University Medical College, 31-501 Krakow, Poland;Department of Medical Diagnostics, Faculty of Pharmacy, Jagiellonian University Medical College, 30-688 Krakow, Poland;Department of Pathophysiology, Faculty of Medicine, Jagiellonian University Medical College, 31-121 Krakow, Poland;Department of Pathophysiology, Forensic Veterinary Medicine and Administration, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland;Institute of Nuclear Physics, Polish Academy of Sciences, 31-342 Krakow, Poland;

关键词: anorexia nervosa;    activity-based anorexia;    animal model;    eating disorders;    starvation;    hyperactivity;   

DOI  :  10.3390/nu13082876

来源: DOAJ

【 摘 要 】

Anorexia nervosa (AN) causes the highest number of deaths among all psychiatric disorders. Reduction in food intake and hyperactivity/increased anxiety observed in AN are also the core features of the activity-based anorexia animal model (ABA). Our aim was to assess how the acute ABA protocol mimics common AN complications, including gonadal and cardiovascular dysfunctions, depending on gender, age, and initial body weight, to form a comprehensive description of ABA as a reliable research tool. Wheel running, body weight, and food intake of adolescent female and male rats were monitored. Electrocardiography, heart rate variability, systolic blood pressure, and magnetic resonance imaging (MRI) measurements were performed. Immediately after euthanasia, tissue fragments and blood were collected for further analysis. Uterine weight was 2 times lower in ABA female rats, and ovarian tissue exhibited a reduced number of antral follicles and decreased expression of estrogen and progesterone receptors. Cardiovascular measurements revealed autonomic decompensation with prolongation of QRS complex and QT interval. The ABA model is a reliable research tool for presenting the breakdown of adaptation mechanisms observed in severe AN. Cardiac and hormonal features of ABA with underlying altered neuroendocrine pathways create a valid phenotype of a human disease.

【 授权许可】

Unknown