Identification of Viral Signatures Using High-Throughput Sequencing on Blood of Patients With Kawasaki Disease

Frontiers in Pediatrics

Noemie Wagner¹  Klara M. Posfay-Barbe¹  Arnaud G. L'Huillier¹  Evgeny Zdobnov²  Francisco Brito³  Laurent Kaiser³  Samuel Cordey⁴ 

[1]Faculty of Medicine, University of Geneva, Geneva, Switzerland

[2]

[3]Division of Pediatric Infectious Diseases, Department of Pediatrics, University Hospitals of Geneva &

[4]Laboratory of Virology, Division of Infectious Diseases and Division of Laboratory Medicine, University Hospitals of Geneva &Swiss Institute of Bioinformatics, Geneva, Switzerland

关键词: Kawasaki disease;    etiology;    high-throughput sequencing;    viral infection;    virus;   

DOI  :  10.3389/fped.2019.00524

来源: DOAJ

【 摘 要 】

Aims: Kawasaki disease is an acute pediatric vasculitis whose etiology remains unknown but epidemiology and clinical presentation suggest a viral etiology. We performed unbiased high-throughput-sequencing on blood of patients with Kawasaki Disease (KD).Materials and Methods: High-throughput-sequencing was performed directly on blood of children with typical KD. Sequences were aligned against a database of clinically relevant viruses.Results: Four patients were acutely infected in the blood, with respectively, poliovirus (vaccine strain), measles (vaccine strain), rhinovirus and bocavirus. Patients with poliovirus and measles had received oral polio and measles vaccines, respectively, twelve and 2 weeks prior.Conclusion: Viral signatures were identified in more than half of the patients, including some corresponding to their vaccinal history. This could suggest a temporal association with KD.

【 授权许可】

Unknown