The miRNAome of Opisthorchis viverrini induced intrahepatic cholangiocarcinoma

Genomics Data

Jordan L. Plieskatt¹  Yanjun Feng¹  Jeffrey M. Bethony¹  Gabriel Rinaldi¹  Jin Peng¹  Paul J. Brindley¹  Therawach Laha²  Samantha E. Easley³  Vajarabhongsa Bhudhisawasdi⁴  Chawalit Pairojkul⁴  Banchob Sripa⁴  Jason P. Mulvenna⁵  Ponlapat Yonglitthipagon⁵ 

[1] Department of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, The George Washington University, Washington, DC 20037, USA;Department of Parasitology, Khon Kaen University School of Medicine, Khon Kaen, 40002, Thailand;Department of Pathology, School of Medicine & Health Sciences, The George Washington University, Washington, DC 20037, USA;Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand;Infectious Diseases Program, QIMR Berghofer Medical Research Institute, Brisbane, Australia;

关键词: MicroRNA;    Cholangiocarcinoma;    Intrahepatic cholangiocarcinoma;    Opisthorchis viverrini;    Microarray;   

DOI  :  10.1016/j.gdata.2014.08.007

来源: DOAJ

【 摘 要 】

Intrahepatic cholangiocarcinoma (ICC) is an aggressive cancer, arising in the biliary ducts that extend into the liver. The highest incidence of ICC occurs in Southeast Asia, particularly in the Mekong River Basin countries of Thailand, Laos, Cambodia, and Vietnam, where it is strongly associated with chronic infection by the food-borne liver fluke Opisthorchis viverrini (OV), one of only three eukaryote pathogens considered Group one carcinogens. Intrahepatic cholangiocarcinoma is usually diagnosed at an advanced stage, with a poor prognosis and survival often less than 24 months. Hence, biomarkers that enable the early detection of ICC would be desirable and have a potentially important impact on the public health in the resource-poor regions where this cancer is most prevalent. As microRNAs (miRNAs) remain well preserved after formalin fixation, there is much interest in developing them as biomarkers that can be investigated using tumor biopsy samples preserved in formalin fixed paraffin embedded (FFPE) tumor blocks. Recently, we reported the first comprehensive profiling of tissue-based miRNA expression using FFPE from the three most common subtypes of OV-induced ICC tumors: moderately differentiated ICC, papillary ICC, and well-differentiated ICC. We observed that each subtype of OV-induced ICC exhibited a distinct miRNA profile, which suggested the involvement of specific sets of miRNAs in the progression of this cancer. In addition, non-tumor tissue adjacent to ICC tumor tissue on the same FFPE block shared a similar miRNA dysregulation profile with the tumor tissue than with normal (non-tumor) liver tissue (individuals without ICC or OV infection). Herein, we provide a detailed description of the microarray analysis procedures used to derive these findings.

【 授权许可】

Unknown