PAX7 Balances the Cell Cycle Progression via Regulating Expression of Dnmt3b and Apobec2 in Differentiating PSCs

Cells

Anita Florkowska¹  Lukasz Truszkowski¹  Maria A. Ciemerych¹  Igor Meszka¹  Magdalena Zawada¹  Iwona Grabowska¹  Monika Granica¹  Zuzanna Jablonska¹  Joanna Nowacka¹ 

[1] Department of Cytology, Institute of Developmental Biology and Biomedical Sciences, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland;

关键词: mouse;    ESCs;    iPSCs;    stem cells;    differentiation;    myogenesis;   

DOI  :  10.3390/cells10092205

来源: DOAJ

【 摘 要 】

PAX7 transcription factor plays a crucial role in embryonic myogenesis and in adult muscles in which it secures proper function of satellite cells, including regulation of their self renewal. PAX7 downregulation is necessary for the myogenic differentiation of satellite cells induced after muscle damage, what is prerequisite step for regeneration. Using differentiating pluripotent stem cells we documented that the absence of functional PAX7 facilitates proliferation. Such action is executed by the modulation of the expression of two proteins involved in the DNA methylation, i.e., Dnmt3b and Apobec2. Increase in Dnmt3b expression led to the downregulation of the CDK inhibitors and facilitated cell cycle progression. Changes in Apobec2 expression, on the other hand, differently impacted proliferation/differentiation balance, depending on the experimental model used.

【 授权许可】

Unknown