Efecto del tiempo y la temperatura en la viabilidad del ADN en la perfilación genética de muestras de sangre/Effects of time and temperature in the viability of DNA in genetic profiling of blood samples

Archivos de Criminología, Seguridad Privada y Criminalística

Christian Ramón Hernández Sánchez¹ 

[1] Laboratorio GENTEST;

关键词: Viabilidad;    ADN;    Sangre;    Temperatura;    Amplificación;    Viability;    DNA;    Blood;    Genetic profile;    Temperature;    Amplification;   

DOI  :  

来源: DOAJ

【 摘 要 】

In this study has been investigated the feasibility of obtaining DNA genetic profiling of biological samples from male human blood subject temperature and humidity factors. The methodology consisted of a sample preparation, DNA extraction, PCR amplification of genetic marker Amelogenin and finally DNA sequencing, we determined the incidence of effective amplification to obtain a complete profile, partial or no sample problem. Furthermore human blood samples over eight days of exposure showed a lower amplification. This research seeks to level the playing field having a scene, the type of samples found, so that the information gathered in this research is very useful trying to help establish viable which samples are to be analyzed in the laboratory.

【 授权许可】

Unknown