Serological investigation of Gyrovirus homsa1 infections in chickens in China

BMC Veterinary Research

Yubao Li¹  Gen Li²  Ruiqi Li³  Defang Zhou³  Shiyu Yuan³  Ziqiang Cheng³  Shicheng Zhang³  Tianxing Yan³  Xiaojing Hao⁴ 

[1]Agricultural Science and Engineering School, Liaocheng University

[2]College of Veterinary Medicine, Qingdao Agricultural University

[3]College of Veterinary Medicine, Shandong Agricultural University

[4]Qingdao Animal Husbandry Workstation (Qingdao Animal Husbandry and Veterinary Research Institute)

关键词: Gyrovirus homsa1;    Seroepidemiology;    Indirect ELISA;    Chicken;   

DOI  :  10.1186/s12917-022-03334-0

来源: DOAJ

【 摘 要 】

Abstract Background Gyrovirus homsa1 (GyH1) (also known as Gyrovirus 3, GyV3) is a non-enveloped, small, single-stranded DNA virus, which was first identified in children with acute diarrhea, and was subsequently detected in marketed chickens, broilers with transmissible viral proventriculitis (TVP), and mammals. GyH1 is a pathogenic virus in chickens, causing aplastic anemia, immunosuppression, and multisystem damage. However, the seroepidemiology of GyH1 infection in chickens remains unclear. Here, we investigated the seroprevalence of GyH1 in chickens by ELISA to reveal the endemic status of GyH1 in China. Results An indirect ELISA with high sensitivity and specificity was developed for investigation of seroepidemiology of GyH1 in chickens in China. The seropositive rate of GyH1 ranged from 0.6% to 7.7% in thirteen provinces, and ranged from 4.1% to 8.1% in eight species chickens. The seropositive rate of GyH1 in broiler breeders was significantly higher than that of in layers. There was a negative correlation between seropositive rate and age of chickens. The highest and lowest seropositive rate were present in chickens at 30–60 days and over 180 days, respectively. Conclusions The seroepidemiological investigation results demonstrated that natural GyH1 infection is widespread in chickens in China. Different species showed different susceptibility for GyH1. Aged chickens showed obvious age-resistance to GyH1. GyH1 has shown a high risk to the poultry industry and should be highly concerned.

【 授权许可】

Unknown