Molecular Characterization of Sarcocystis Species Isolated from Sheep and Goats in Riyadh, Saudi Arabia

Animals

MashaelA. Al-Damigh¹  IsraM. Al-Turaiki²  DinaM. Metwally³  ManalF. El-Khadragy³ 

[1] Biology Department, Faculty of Education, Al-Majmaah University, Al-Majmaah 11952, Saudi Arabia;Department of Information Technology, Faculty of Computer and Information Science, King Saud University, Riyadh 11451, Saudi Arabia;Zoology Department, Faculty of Science, King Saud University, Riyadh 11451, Saudi Arabia;

关键词: Sarcocystis species;    COX1;    sheep and goats;   

DOI  :  10.3390/ani9050256

来源: DOAJ

【 摘 要 】

Sarcocystosis is induced by species of Sarcocystis, which is an intracellular protozoan parasite in the phylum Apicomplexa. The diversity and importance of Sarcocystis species in sheep and goats in Saudi Arabia are poorly understood. In this study, the tongue, esophagus, heart, diaphragm, and skeletal muscles were collected from 230 sheep and 84 goats, and the tissues were examined for the presence of Sarcocystis species by macroscopic examination and light microscopy. Microscopic Sarcocystis species cysts were found in both sheep and goats. Transmission electron microscopy (TEM) revealed S. tenella in sheep and S. capracanis in goats. Sarcocystis species were confirmed for the first time in Saudi Arabian sheep and goats by molecular testing. S. capracanis was most closely related to S. tenella, with the COX1 sequences sharing 91.7% identity. A phylogenetic analysis produced similar results and indicated that the Sarcocystis isolates were within a group of Sarcocystis species in which dogs were the final host. Finally, the Sarcocystis species cysts from sheep and goats could be grouped together, indicating that they were strongly related.

【 授权许可】

Unknown