Evaluation of dietary linoleic acid on growth as well as hepatopancreatic index, lipid accumulation oxidative stress and inflammation in Chinese mitten crabs (Eriocheir sinensis)

Aquaculture Reports

Wen-Bin Liu¹  Guang-Zhen Jiang²  Cheng Chi²  Ding-Dong Zhang²  Kenneth Prudence Abasubong²  Yong-Jun Dai²  Xiang-Fei Li² 

[1] Corresponding author.;Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, National Experimental Teaching Center for Animal Science, College of Animal Science and Technology, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, Jiangsu Province, People’s Republic of China;

关键词: Eriocheir sinensis;    Growth;    Linoleic acid;    Hepatopancreatic index;    Oxidative stress;   

DOI  :  

来源: DOAJ

【 摘 要 】

This study aimed to investigate the effect of dietary linoleic acid (LA) on the growth, hepatopancreatic development and health of Eriocheir sinensis. Crabs (initial body weight: 28.53 ± 0.63 g) were fed with five formulated diets containing graded levels of LA (7, 14, 21, 28, 35 g/kg) for 90 days. Each diet was tested in four replicates. A significant (P < 0.05) increase in weight gain (WG) and specific growth rate (SGR) was observed as dietary LA levels vary 21–35 g/kg. 21 or 28 g/kg dietary LA promoted hepatopancreatic development, which was evidenced by an increased (P < 0.05) hepatopancreatic index (HSI) and decreased (P < 0.05) proportion of G2/M cells. Immunofluorescence analysis showed that the accumulation of hepatopancreatic lipid increased (P < 0.05) in 21 and 28 g/kg LA groups. These effects possibly were associated with increased (P < 0.05) expression of CPT1, ACO, FATP 4, FABP10 and FAS. In addition, increased (P < 0.05) malondialdehyde (MDA) and suppressed (P < 0.05) total antioxidant capacity (T-AOC) was observed in E. sinensis fed with 28 or 35 28 g/kg dietary LA. The expression of genes related to inflammation showed no significant difference among all groups. In conclusion, 21 or 28 g/kg dietary LA promoted growth, HSI and the accumulation of hepatopancreatic lipid; High-grade dietary LA (28 and 35 g/kg) did not induce inflammation and inhibit growth but posed a threat to oxidative stress.

【 授权许可】

Unknown