Immune Trait Shifts in Association With Tobacco Smoking: A Study in Healthy Women

Frontiers in Immunology

Alessio Naccarati¹  Simona Rolla²  Giulia Piaggeschi³  Francesca Cordero³  Niccolò Rossi⁴  Simon Couvreur⁴  Mario Falchi⁴  Tim D. Spector⁴  Alessia Visconti⁴  Davide Brusa⁵  Massimo Mangino⁷  Mario Roederer⁸ 

[1] Candiolo Cancer Institute, Fondazione del Piemonte per l'Oncologia-Istituto di Ricovero e Cura a Carattere Scientifico (FPO-IRCCS), Turin, Italy;Department of Clinical and Biological Sciences, University of Turin, Turin, Italy;Department of Computer Science, University of Turin, Turin, Italy;Department of Twin Research and Genetic Epidemiology, King's College London, London, United Kingdom;Institute of Experimental and Clinical Research, Université Catholique de Louvain, Brussels, Belgium;Italian Institute for Genomic Medicine, c/o IRCCS Candiolo, Turin, Italy;National Institute for Health Research (NIHR) Biomedical Research Centre at Guy's and St Thomas' Foundation Trust, London, United Kingdom;Vaccine Research Center, National Institutes of Health, Bethesda, MD, United States;

关键词: tobacco smoking exposure;    immune traits;    leukocyte-shift;    immune cell subset frequencies;    epidemiology;   

DOI  :  10.3389/fimmu.2021.637974

来源: DOAJ

【 摘 要 】

Tobacco smoking is known to impact circulating levels of major immune cells populations, but its effect on specific immune cell subsets remains poorly understood. Here, using high-resolution data from 223 healthy women (25 current and 198 never smokers), we investigated the association between smoking status and 35,651 immune traits capturing immune cell subset frequencies. Our results confirmed that active tobacco smoking is associated with increased frequencies of circulating CD8+ T cells expressing the CD25 activation marker. Moreover, we identified novel associations between smoking status and relative abundances of CD8+ CD25+ memory T cells, CD8+ memory T cells expressing the CCR4 chemokine receptor, and CD4+CD8+ (double-positive) CD25+ T cells. We also observed, in current smokers, a decrease in the relative frequencies of CD4+ T cells expressing the CD38 activation marker and an increase in class-switched memory B cell isotypes IgA, IgG, and IgE. Finally, using data from 135 former female smokers, we showed that the relative frequencies of immune traits associated with active smoking are usually completely restored after smoking cessation, with the exception of subsets of CD8+ and CD8+ memory T cells, which persist partially altered. Our results are consistent with previous findings and provide further evidence on how tobacco smoking shapes leukocyte cell subsets proportion toward chronic inflammation.

【 授权许可】

Unknown