Identification and Characterization of Triple Action Bioagents (TAB) and Their Potency against Fusarium Wilt of Lentil

Horticulturae

Xiukang Wang¹  Ayman EL Sabagh²  Muntazir Mushtaq³  H. S. Viswanath⁴  Raheeba Tun Nisa⁴  Shaheen Kauser Jan⁴  Farooq Ahmad bhat⁴  Khurshid Ahmad Bhat⁴  Umer Basu⁵  Muhammad Habib ur Rahman⁶  Walid Soufan⁷  Rameesa Rashid⁸ 

[1] College of Life Sciences, Yan’an University, Yan’an 716000, China;Department of Agronomy, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh 33516, Egypt;Division of Germplasm Evaluation, ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110012, India;Division of Plant Pathology, FoA, Sher-e-Kashmir University of Agricultural Sciences and Technology-Kashmir, Wadura Sopore, Srinagar 190025, India;Division of Plant Pathology, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Jammu 18009, India;Institute of Crop Science and Resource Conservation (INRES), University of Bonn, 53113 Bonn, Germany;Plant Production Department, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia;Regional Research Institute of Unani Medicine Srinagar, Srinagar 190003, India;

关键词: bio-agents;    copper oxychloride;    phosphorus solubilization;    Fusarium wilt;    lentil;   

DOI  :  10.3390/horticulturae7120587

来源: DOAJ

【 摘 要 】

Fusarium wilt is a severe disease that plays a significant role in reducing the yield of lentil. Under favorable conditions for disease growth, the disease can cause complete crop failure and can be a crucial limiting issue for lentil cultivation in specific geographical zones. The current work focused on isolating potentialbio-agents exhibiting copper oxychloride resistance and evaluating their efficacy in seed treatment for ecologically sustainable management of Fusarium wilt of lentil. Seventy biocontrol agent isolates were isolated and tested for resistance by growing them on Potato Dextrose Agar medium (PDA) amended with copper oxychloride at the rate of 2500 ppm. Isolate-H10 and isolate-C9 showed more excellent compatibility with copper oxychloride fungicide with 69 mm and 65 mm radial growths, respectively. The isolates H10 and C9 had the highest inhibitory percentages of 84.30% and 83.94% against Fusarium oxysporum f. sp. lentis, respectively, and the highest phosphorus solubilization index (PSI). Primers (ITS 1 and ITS 4) identified these putative bioagents as Trichoderma harzianum isolate skua-tab-1 and Penicillium crysogenum strain Tab2. Sequences were submitted to the NCBI and assigned the accession numbers MK414603 and MK418066. In pot culture, these isolates also demonstrated their superiority in reducing the disease incidence and severity if seeds were treated with H10 and C9 alone or in combination with copper oxychloride fungicide. The two isolated bioagents exhibit three fundamental properties: compatibility with copper oxychloride, antagonistic activity toward the pathogen fall armyworm, and the ability to dissolve phosphorus minerals.

【 授权许可】

Unknown