Recombinase-Aided Amplification Coupled with Lateral Flow Dipstick for Efficient and Accurate Detection of Porcine Parvovirus

Life

Jinding Chen¹  Wenxian Chen¹  Lin Yi¹  Jindai Fan¹  Hongxing Ding¹  Yihong He¹  Shuangqi Fan¹ 

[1] College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;

关键词: porcine parvovirus;    recombinase-aided amplification;    lateral flow dipstick;   

DOI  :  10.3390/life11080762

来源: DOAJ

【 摘 要 】

Porcine parvovirus (PPV) infection is the primary cause of SMEDI (stillbirth; mummification; embryonic death; infertility) syndrome, which is a global burden for the swine industry. Thus, it is crucial to establish a rapid and efficient detection method against PPV infection. In the present work, we developed a recombinase-aided amplification (RAA) assay, coupled with a lateral flow dipstick (LFD), to achieve an amplification of PPV DNA at 37 °C within 15 min. The detection limits of PPV RAA-LFD assay were 10² copies/μL recombinant plasmid pMD19-T-VP1, 6.38 × 10⁻⁷ ng/μL PPV DNA, and 10⁻¹ TCID₅₀/mL virus, respectively. This method was highly specific for PPV detection with no cross-reactivity for other swine pathogens. In contrast to polymerase chain reaction (PCR), the PPV RAA-LFD assay is more sensitive and cost-saving. Hence, the established PPV RAA-LFD assay provided an alternative for PPV detection, especially in resource-limited regions.

【 授权许可】

Unknown