Leucine Promotes Proliferation and Differentiation ofPrimary Preterm Rat Satellite Cells in Part throughmTORC1 Signaling Pathway

Nutrients

Chu-Yi Guo¹  Zhen-Yu Shen¹  Xiao-Shan Qiu¹  Mu-Xue Yu¹  Jie-Min Dai¹  Si-Qi Zhuang¹ 

[1] Department of Pediatrics, The First Affiliated Hospital, Sun Yat-sen University,Guangzhou, Guangdong Province 510080, China;

关键词: leucine;    preterm;    satellite cells;    proliferation;    differentiation;    mTOR;    MyoD;    myogenin;   

DOI  :  10.3390/nu7053387

来源: DOAJ

【 摘 要 】

Signaling through the mammalian target of rapamycin (mTOR) in response to leucine modulates many cellular and developmental processes. However, in the context of satellite cell proliferation and differentiation, the role of leucine and mTORC1 is less known. This study investigates the role of leucine in the process of proliferation and differentiation of primary preterm rat satellite cells, and the relationship with mammalian target of rapamycin complex 1 (mTORC1) activation. Dissociation of primary satellite cells occurred with type I collagenase and trypsin, and purification, via different speed adherence methods. Satellite cells with positive expression of Desmin were treated with leucine and rapamycin. We observed that leucine promoted proliferation and differentiation of primary satellite cells and increased the phosphorylation of mTOR. Rapamycin inhibited proliferation and differentiation, as well as decreased the phosphorylation level of mTOR. Furthermore, leucine increased the expression of MyoD and myogenin while the protein level of MyoD decreased due to rapamycin. However, myogenin expressed no affect by rapamycin. In conclusion, leucine may up-regulate the activation of mTORC1 to promote proliferation and differentiation of primary preterm rat satellite cells. We have shown that leucine promoted the differentiation of myotubes in part through the mTORC1-MyoD signal pathway.

【 授权许可】

Unknown