Quantification of torque teno virus (TTV) DNA in saliva and plasma samples in patients at short time before and after kidney transplantation

Journal of Oral Microbiology

José Medina-Pestana¹  Louise Zanella²  Maria A. Stincarelli³  Simone Giannecchini³  Bengt Hasséus⁴  Rafael A. V. Caixeta⁵  Dmitry J. S. Sarmento⁵  Marina Gallottini⁵  Matheus W. Caetano⁶  Ana C. Mamana⁶  Rodrigo Melim Zerbinati⁶  Tania R. Tozetto-Mendoza⁶  Alexandre Mendes Batista⁶  Paulo H. Braz-Silva⁶ 

[1] Federal University of São Paulo School of Medicine;Universidad de La Frontera;University of Florence;University of Gothenburg Institute of Odontology;University of São Paulo School of Dentistry;University of São Paulo School of Medicine;

关键词: saliva;    immunosuppression;    solid organ transplantation;    torque teno virus;   

DOI  :  10.1080/20002297.2021.2008140

来源: DOAJ

【 摘 要 】

Background Several reports have proposed that the viral load of torque teno virus (TTV) in plasma is a biomarker of immune function in solid organ transplantation (SOT) and in allogeneic hematopoietic stem cell transplantation. Additionally, for the latter one, TTV-DNA quantification in saliva has also been suggested. Aim to investigate the correlation between the TTV viral load and immune function in paired saliva and plasma samples in patients on kidney transplantation. Materials and Methods TTV-DNA viral load was quantified in paired samples of saliva and plasma from 71 patients before and a short-time after renal-transplantation by real-time PCR. Results The data obtained from 213 paired samples showed a slight consistency in the comparison between saliva and plasma, with prevalence of TTV-DNA being 58%, 52% and 60% in saliva samples and 60%, 73% and 90% in plasma samples before and at 15–20 and 45–60 days after transplantation, respectively. Additionally, a high TTV viral load was observed in plasma at 15–20 and 45–60 days after transplantation compared to that observed in saliva at the same time. Conclusions Overall, monitoring TTV-DNA in saliva samples could be an additional fast non-invasive option to assess the immune functionality in SOT populations.

【 授权许可】

Unknown