Extraction and Evaluation of Bioactive Compounds from Date (Phoenix dactylifera) Seed Using Supercritical and Subcritical CO2 Techniques

Foods

Kashif Ghafoor¹  Abdullah K. Almubarak¹  Mohammed S. Alkaltham¹  Fahad Y. Al-Juhaimi¹  Elfadil E. Babiker¹  Md. Zaidul Islam Sarker² 

[1] Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia;Food Science Program, Cooperative Research, Extension and Education Services, Northern Marianas College, Saipan, MP 96950, USA;

关键词: Phoenix dactylifera;    date fruit seed;    supercritical fluid;    subcritical CO₂;    phenolics;    carotenoids;   

DOI  :  10.3390/foods11121806

来源: DOAJ

【 摘 要 】

Date (Phoenix dactylifera) seed is a potential source of natural antioxidants, and the use of innovative green and low temperature antioxidant recovery techniques (using CO₂ as solvent) such as supercritical fluid (SFE) and subcritical (SubCO₂) extractions can improve their yields and quality in the extracts. SFE, SubCO₂ and Soxhlet techniques were employed to enrich antioxidants in extracts from Sukari (SKSE), Ambara (AMSE), Majdool (MJSE) and Sagai (SGSE) date seeds. Extract yields were evaluated and modelled for SFE extract using response surface methodology. Significantly higher (p < 0.05) phenolics (143.48–274.98 mg GAE/100 g), flavonoids (78.35–141.78 mg QE/100 g), anthocyanins (0.39–1.00 mg/100 g), and carotenoid (1.42–1.91 mg BCE/100 g) contents were detected in extracts obtained using SFE and SubCO₂ methods. The evaluation of in vitro antioxidant properties showed that SFE and SubCO₂ seed extracts demonstrated promising antioxidant (13.42–23.83 µg AAE/mL), antiradical (228.76–109.69 µg/mL DPPH IC₅₀), ferric reducing antioxidant power (1.43–2.10 mmol TE/100 g) and ABTS cation scavenging (375.74-717.45 µmol TE/100 g) properties that were significantly higher than Soxhlet extracts. Both SFE and SubCO₂ techniques can be effectively utilized as innovative and environmentally friendly alternatives to obtain high quality antioxidant rich extracts from date seed. These extracts may have potential functional and nutraceutical applications.

【 授权许可】

Unknown