Visualization of the Crossroads between a Nascent Infection Thread and the First Cell Division Event in Phaseolus vulgaris Nodulation

International Journal of Molecular Sciences

Raúl Dávila-Delgado¹  Emmanuel Ayala-Guzmán¹  Elizabeth Monroy-Morales¹  Rosana Sánchez-López¹  Alicia Gamboa-deBuen² 

[1] Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Colonia Chamilpa, Cuernavaca 62210, Mexico;Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad de México 04510, Mexico;

关键词: cell division;    cell plate;    cytokinesis;    infection thread;    nodulation;    nucleus;   

DOI  :  10.3390/ijms23095267

来源: DOAJ

【 摘 要 】

The development of a symbiotic nitrogen-fixing nodule in legumes involves infection and organogenesis. Infection begins when rhizobia enter a root hair through an inward structure, the infection thread (IT), which guides the bacteria towards the cortical tissue. Concurrently, organogenesis takes place by inducing cortical cell division (CCD) at the infection site. Genetic analysis showed that both events are well-coordinated; however, the dynamics connecting them remain to be elucidated. To visualize the crossroads between IT and CCD, we benefited from the fact that, in Phaseolus vulgaris nodulation, where the first division occurs in subepidermal cortical cells located underneath the infection site, we traced a Rhizobium etli strain expressing DsRed, the plant cytokinesis marker YFP-PvKNOLLE, a nuclear stain and cell wall auto-fluorescence. We found that the IT exits the root hair to penetrate an underlying subepidermal cortical (S-E) cell when it is concluding cytokinesis.

【 授权许可】

Unknown