The Effect of Donor Human Milk Fortification on The Adhesion of Probiotics In Vitro

Nutrients

Samuli Rautava¹  Erika Isolauri¹  ArturC. Ouwehand²  Anastasia Mantziari³  Satu Tölkkö³  Seppo Salminen³  Eliisa Löyttyniemi⁴ 

[1] Department of Pediatrics, University of Turku and Turku University Hospital, Kiinamyllynkatu 4-8, 20520 Turku, Finland;DuPont Nutrition and Biosciences, Sokeritehtaantie 20, 02460 Kantvik, Finland;Functional Foods Forum, Faculty of Medicine, University of Turku, Itäinen Pitkäkatu 4A, 20520 Turku, Finland;Unit of Biostatistics, Department of Clinical Medicine University of Turku, Kiinamyllynkatu 10, 20520 Turku, Finland;

关键词: human milk fortifier;    infant nutrition;    preterm infant;    lactobacillus rhamnosus gg;    bifidobacterium animalis subsp. lactis bb12;   

DOI  :  10.3390/nu12010182

来源: DOAJ

【 摘 要 】

Preterm delivery complications are the primary cause of death among children under the age of five. Preventive strategies include the use of pasteurized donor human milk (DHM), its fortification with human milk fortifiers (protein supplements), and supplementation with probiotics. Our aim was to examine the impact of DHM and fortified DHM (FDHM) on the mucus adhesion properties of two widely used probiotics. The study covered two forms of human milk fortifier, liquid and powdered, with or without probiotics and storage at 4 °C for 24 h. To test the adhesion properties of the probiotic strains, DHM+probiotics and FDHM+probiotics were prepared and added to immobilized mucus isolated from the stool of healthy Finnish infants. The probiotic adhesion was then measured by liquid scintillation. Our results suggest that addition of liquid or powdered human milk fortifier in donor human milk had no impact on probiotic adhesion. In addition, given the increased adhesion of probiotics suspended in buffer, other matrices should be further studied. These factors need to be considered when designing future intervention strategies using probiotics in preterm infants.

【 授权许可】

Unknown