Morphological and Proteomic Analyses Reveal that Unsaturated Guluronate Oligosaccharide Modulates Multiple Functional Pathways in Murine Macrophage RAW264.7 Cells

Marine Drugs

Xu Xu¹  Li-Ming Shen¹  De-Cheng Bi¹  Chao Li¹  Wei-Shan Fang¹  Rui Zhou¹  Shui-Ming Li²  Min Wan³  Lian-Li Chi⁴ 

[1] College of Life Science, Shenzhen Key Laboratory of Marine Bioresources and Ecology,Shenzhen University, Shenzhen 518060, China;College of Life Science, Shenzhen Key Laboratory of Microbial Genetic Engineering,Shenzhen University, Shenzhen 518060, China;Division of Physiological Chemistry 2, Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm 17177, Sweden;NationalGlycoengineering Research Center, Shandong University, Jinan 250100, China;

关键词: guluronate oligosaccharide;    macrophage activation;    nuclear factor-κB;    anti-inflammation;    antioxidant;    cell morphology;   

DOI  :  10.3390/md13041798

来源: DOAJ

【 摘 要 】

Alginate is a natural polysaccharide extracted from various species of marine brown algae. Alginate-derived guluronate oligosaccharide (GOS) obtained by enzymatic depolymerization has various pharmacological functions. Previous studies have demonstrated that GOS can trigger the production of inducible nitric oxide synthase (iNOS)/nitric oxide (NO), reactive oxygen species (ROS) and tumor necrosis factor (TNF)-α by macrophages and that it is involved in the nuclear factor (NF)-κB and mitogen-activated protein (MAP) kinase signaling pathways. To expand upon the current knowledge regarding the molecular mechanisms associated with the GOS-induced immune response in macrophages, comparative proteomic analysis was employed together with two-dimensional electrophoresis (2-DE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF MS) and Western blot verification. Proteins showing significant differences in expression in GOS-treated cells were categorized into multiple functional pathways, including the NF-κB signaling pathway and pathways involved in inflammation, antioxidant activity, glycolysis, cytoskeletal processes and translational elongation. Moreover, GOS-stimulated changes in the morphologies and actin cytoskeleton organization of RAW264.7 cells were also investigated as possible adaptations to GOS. This study is the first to reveal GOS as a promising agent that can modulate the proper balance between the pro- and anti-inflammatory immune responses, and it provides new insights into pharmaceutical applications of polysaccharides.

【 授权许可】

Unknown