Partial purification and characterization of a thermostable xylanase from Bacillus licheniformis isolated from hot water geyser

Journal of Genetic Engineering and Biotechnology

Shilpa S. Chapadgaonkar¹  Girisha Malhotra² 

[1] MIT World Peace University;Manav Rachna International Institute of Research and Studies;

关键词: Xylanase;    Thermostable;    Bacillus;    Enzyme purification;    Enzyme characterization;    Gel filtration chromatography;   

DOI  :  10.1186/s43141-022-00333-4

来源: DOAJ

【 摘 要 】

Abstract Background Thermo-alkali stable xylanases were purified from the extracellular broth of newly isolated Bacillus licheniformis strain produced in a 5-L stirred-tank bioreactor with wheat bran as a carbon source. Results A high degree of purity was achieved using size exclusion chromatography resulting in 16-fold purification and 69% recovery for fraction 5 which had the highest activity. The recovery obtained after pooling fractions 5 and 6 was 99%. The K m value of xylanase was calculated as 0.05 mM, and V max was 125 μmol/min/mg protein. Conclusion Purified xylanase had a high thermal and pH stability. Xylanase was found to be suitable for application in the de-inking of paper and for saccharification of lignocellulosic waste biomass.

【 授权许可】

Unknown