In Situ Flow Cytometer Calibration and Single-Molecule Resolution via Quantum Measurement

Sensors

Javier Sabines-Chesterking¹  Ivan A. Burenkov¹  Sergey V. Polyakov² 

[1] Joint Quantum Institute, University of Maryland, College Park, MD 20742, USA;National Institute of Standards and Technology, Gaithersburg, MD 20899, USA;

关键词: flow cytometry;    single-molecule;    single-photon sources;   

DOI  :  10.3390/s22031136

来源: DOAJ

【 摘 要 】

Fluorescent biomarkers are used to detect target molecules within inhomogeneous populations of cells. When these biomarkers are found in trace amounts it becomes extremely challenging to detect their presence in a flow cytometer. Here, we present a framework to draw a detection baseline for single emitters and enable absolute calibration of a flow cytometer based on quantum measurements. We used single-photon detection and found the second-order autocorrelation function of fluorescent light. We computed the success of rare-event detection for different signal-to-noise ratios (SNR). We showed high-accuracy identification of the events with occurrence rates below ${10}^{-5}$ even at modest SNR levels, enabling early disease diagnostics and post-disease monitoring.

【 授权许可】

Unknown