RNA Interference Suppression of v-ATPase B and Juvenile Hormone Binding Protein Genes Through Topically Applied dsRNA on Tomato Leaves: Developing Biopesticides to Control the South American Pinworm, Tuta absoluta (Lepidoptera: Gelechiidae)

Frontiers in Physiology

Patcharin Krutmuang¹  Mona S. Alwahibi²  Mohamed Soliman Elshikh²  Ahmed Abdel-Megeed⁴  Aml Ghaith⁵  Sengodan Karthi⁶  Sengottayan Senthil-Nathan⁶  Govindaraju Ramkumar⁷  B. Kariyanna⁷  Ramasamy Asokan⁷  N. R. Prasannakumar⁸  Kandaswamy Kalaivani⁹  Wayne Brian Hunter^1,10 

[1] 0Innovative Agriculture Research Center, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand;Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia;Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai, Thailand;Department of Plant Protection, Faculty of Agriculture Saba Basha, Alexandria University, Alexandria, Egypt;Department of Zoology, Faculty of Science, Derna University, Derna, Libya;Division of Biopesticides and Environmental Toxicology, Sri Paramakalyani Center for Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Tirunelveli, India;Division of Biotechnology, ICAR-Indian Institute of Horticultural Research (IIHR), Bengaluru, India;Division of Entomology and Nematology, ICAR-Indian Institute of Horticultural Research (IIHR), Bengaluru, India;Post Graduate and Research Center, Department of Zoology, Sri Parasakthi College for Women, Tirunelveli, India;U.S. Horticultural Research Laboratory, United States Department of Agriculture, Agricultural Research Service, Fort Pierce, FL, United States;

关键词: tomato leaf miner;    v-ATPase;    juvenile hormone binding protein;    JHBP;    dsRNA;    RNAi;   

DOI  :  10.3389/fphys.2021.742871

来源: DOAJ

【 摘 要 】

The South American pinworm Tuta absoluta (Meyrick) (Family: Gelechiidae) is one of the most devastating lepidopteran pests in the developing countries of South America, Africa, and Asia. This pest is classified as the most serious threat for tomato production worldwide. In the present study, we analyzed RNAi-mediated control through exogenously applied dsRNA delivery on tomato. The dsRNA treatments were made to target the juvenile hormone binding protein and the v-ATPase B. Both mRNA targets were cloned, validated by sequencing, and used to produce each dsRNA. After treatments the relative transcript expression was analyzed using qRTPCR to assess to efficacy of RNAi. A leaf-dip assay was used to provide late 2nd instar larvae three feeding access periods: 24, 48, and 72 h, to evaluate the effect of gene silencing of each target. Larvae were fed tomato leaves coated with five different RNAi concentrations (10, 20, 30, 40, and 50 micrograms/centimeter-squared), that suppressed two genes (juvenile hormone protein, JHBP, and vacuolar-type adenosine triphosphatase enzyme, v-ATPase). Treatments with dsRNA showed a significant increase in mortality at 24, 48, and 72 h after ingestion (P < 0.01, α = 0.05), along with reduced leaf damage, and increased feeding deterrence. The results suggest that these two RNAi products may provide a suitable treatment for control of this and other lepidopteran pests.

【 授权许可】

Unknown