Essential Oils of Sage, Rosemary, and Bay Laurel Inhibit the Life Stages of Oomycete Pathogens Important in Aquaculture

Plants

Igor Jerković¹  Zvonimir Marijanović¹  Dorotea Grbin²  Anđela Miljanović²  Dora Pavić²  Maja Dent²  Ana Bielen² 

[1] Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21 000 Split, Croatia;Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10 000 Zagreb, Croatia;

关键词: anti-oomycete activity;    Aphanomyces astaci;    EC₅₀ values;    Mediterranean wild plants;    Saprolegnia parasitica;   

DOI  :  10.3390/plants10081676

来源: DOAJ

【 摘 要 】

Saprolegnia parasitica, the causative agent of saprolegniosis in fish, and Aphanomyces astaci, the causative agent of crayfish plague, are oomycete pathogens that cause economic losses in aquaculture. Since toxic chemicals are currently used to control them, we aimed to investigate their inhibition by essential oils of sage, rosemary, and bay laurel as environmentally acceptable alternatives. Gas Chromatography–Mass Spectrometry (GC–MS) analysis showed that the essential oils tested were rich in bioactive volatiles, mainly monoterpenes. Mycelium and zoospores of A. astaci were more sensitive compared to those of S. parasitica, where only sage essential oil completely inhibited mycelial growth. EC₅₀ values (i.e., concentrations of samples at which the growth was inhibited by 50%) for mycelial growth determined by the radial growth inhibition assay were 0.031–0.098 µL/mL for A. astaci and 0.040 µL/mL for S. parasitica. EC₅₀ values determined by the zoospore germination inhibition assay were 0.007–0.049 µL/mL for A. astaci and 0.012–0.063 µL/mL for S. parasitica. The observed inhibition, most pronounced for sage essential oil, could be partly due to dominant constituents of the essential oils, such as camphor, but more likely resulted from a synergistic effect of multiple compounds. Our results may serve as a basis for in vivo experiments and the development of environmentally friendly methods to control oomycete pathogens in aquaculture.

【 授权许可】

Unknown