Brazilian Consortium for the Study on Renal Diseases Associated With COVID-19: A Multicentric Effort to Understand SARS-CoV-2-Related Nephropathy

Frontiers in Medicine

Lígia Cristina Lopes de Farias¹  Giuseppe Cesare Gatto²  Flávia Lara Barcelos²  Antonio Monteiro³  Igor Denizarde Bacelar Marques⁴  Gustavo Lemos Uliano⁵  Diego do Amaral Polido⁶  Lídio Gonçalves Lima Neto⁷  Carlos Eduardo Campos Maramaldo⁷  Douglas Rafanelle Moura de Santana Motta⁸  Weverton Machado Luchi⁹  Thaísa de Oliveira Leite^1,10  Felipe Leite Guedes^1,11  David Campos Wanderley^1,12  Stanley de Almeida Araújo^1,12  Orlando Vieira Gomes^1,13  Lucila Maria Valente^1,14  Karla Cristina Silva Petruccelli Israel^1,15  Francisco Rasiah Ladchumananandasivam^1,16  Antonio Augusto Lima Teixeira Júnior^1,17  Antônio Fernando Coutinho Filho^1,18  Elieser Hitoshi Watanabe^1,19  Luiz Fernando Onuchic^1,19  Andréia Watanabe^1,19  Gyl Eanes Barros Silva^2,21  Monique Pereira Rêgo Muniz^2,21  Dyego José de Araújo Brito^2,21  Natalino Salgado Filho^2,21  Kaile de Araújo Cunha^2,21  Precil Diego Miranda de Menezes Neves^2,22  Lucas Lobato Acatauassu Nunes^2,24  Joyce Santos Lages^2,25 

[1] 0Medical Clinic Unit, Hospital Universitário Alcides Carneiro (HUAC), Campina Grande, Brazil;0Nephrology Service, University Hospital of Brasilia, University of Brasília (HUB – UNB), Brasília, Brazil;1Maria Aparecida Pedrossian University Hospital, Federal University of Mato Grosso do Sul (HUMAP – UFMS), Campo Grande, Brazil;1Nephrology Service, University Hospital, Federal University of Piauí (HU-UFPI), Teresina, Brazil;2School-Hospital, Federal University of Pelotas (HE-UFPEL), Pelotas, Brazil;2University Hospital, Federal University of Grande Dourados (HU-UFGD), Dourados, Brazil;3Laboratory of Immunology and Microbiology of Respiratory Infections (LIMIR), Maranhão University Center (CEUMA), São Luís, Brazil;3Nephrology Service, University Hospital of the Federal University of Sergipe (HU-UFS), Aracaju, Brazil;4Nephrology Service, Cassiano Antonio Moraes University Hospital, Federal University of Espírito Santo (HUCAM-UFES), Vitória, Brazil;4Nephrology Service, University Hospital of Lizard, Federal University of Sergipe (HUL - UFS), Lagarto, Brazil;5Nephrology Service, Onofre Lopes University Hospital, Federal University of Rio Grande do Norte (HUOL-UFRN), Natal, Brazil;5Nephropathology Institute, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil;6University Hospital, Federal University of Vale do São Francisco (HU-UNIVASF), Petrolina, Brazil;7Faculty of Medicine, Federal University of Pernambuco (UFPE), Recife, Brazil;8Postgraduate Program in Tropical Medicine (FMT/UEA), Department of Clinical Medicine, Federal University of Amazonas (UFAM), Manaus, Brazil;9Nephrology Service, Lauro Wanderley University Hospital, Federal University of Paraíba (HULW-UFPB), João Pessoa, Brazil;Clinical Research Center (CEPEC), University Hospital, Federal University of Maranhão (HU-UFMA), São Luís, Brazil;Dr. Raimundo Bezerra Unit of Dialysis and Kidney Transplantation (UNIRIM), Crato, Brazil;Molecular Medicine Discipline, Medical School, University of São Paulo (FM-USP), São Paulo, Brazil;Nephrology Division, Medical School, University of São Paulo (FM-USP), São Paulo, Brazil;Nephrology Service, University Hospital, Federal University of Maranhão (HU-UFMA), São Luís, Brazil;Nephrology and Dialysis Center, Oswaldo Cruz German Hospital, São Paulo, Brazil;Postgraduate Program in Genetics, Ribeirão Preto Medical School, University of São Paulo (PGGEN-FMRP-USP), Ribeirão Preto, Brazil;University Hospital Complex, João de Barros Barreto University Hospital, Federal University of Pará (HUJBB – UFPA), Belém, Brazil;University Hospital, Federal University of Maranhão (HU-UFMA), São Luís, Brazil;

关键词: COVID-19;    SARS-CoV-2;    glomerulopathy;    kidney injury;    collapsing glomerulopathy;    thrombotic microangiopathy;   

DOI  :  10.3389/fmed.2020.584235

来源: DOAJ

【 摘 要 】

Kidney involvement appears to be frequent in coronavirus disease 2019 (COVID-19). Despite this, information concerning renal involvement in COVID-19 is still scarce. Several mechanisms appear to be involved in the complex relationship between the virus and the kidney. Also, different morphological patterns have been described in the kidneys of patients with COVID-19. For some authors, however, this association may be just a coincidence. To investigate this issue, we propose assessing renal morphology associated with COVID-19 at the renal pathology reference center of federal university hospitals in Brazil. Data will come from a consortium involving 17 federal university hospitals belonging to Empresa Brasileira de Serviços Hospitalares (EBSERH) network, as well as some state hospitals and an autopsy center. All biopsies will be sent to the referral center for renal pathology of the EBSERH network. The data will include patients who had coronavirus disease, both alive and deceased, with or without pre-existing kidney disease. Kidney biopsies will be analyzed by light, fluorescence, and electron microscopy. Furthermore, immunohistochemical (IHC) staining for various inflammatory cells (i.e., cells expressing CD3, CD20, CD4, CD8, CD138, CD68, and CD57) as well as angiotensin-converting enzyme 2 (ACE2) will be performed on paraffinized tissue sections. In addition to ultrastructural assays, in situ hybridization (ISH), IHC and reverse transcription-polymerase chain reaction (RT-PCR) will be used to detect Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV-2) in renal tissue. For the patients diagnosed with Collapsing Glomerulopathy, peripheral blood will be collected for apolipoprotein L-1 (APOL1) genotyping. For patients with thrombotic microangiopathy, thrombospondin type 1 motif, member 13 (ADAMTS13), antiphospholipid, and complement panel will be performed. The setting of this study is Brazil, which is second behind the United States in highest confirmed cases and deaths. With this complete approach, we hope to help define the spectrum and impact, whether immediate or long-term, of kidney injury caused by SARS-CoV-2.

【 授权许可】

Unknown