Rebuilding hippocampus neural circuit with hADSC-derived neuron cells for treating ischemic stroke

Cell & Bioscience

Rui Hao¹  Ying Huang¹  Juan Wang²  Limei Cao³  Tianfang Jiang³  Xu Chen³  Fei Zhou⁴  Ke Ning⁵  Guangming Wang⁶  Xuanxuan Guo⁶  Shane Gao⁶  Jun Xu⁶  Chunlong Zhong⁶  Jian Wang⁶  Fengjuan Gao⁷ 

[1] Center of Translational Medicine, Tongji Hospital, Tongji University School of Medicine;Department of Biotechnology and Molecular, Binzhou Medical College;Department of Neurology, Shanghai Eighth People’s Hospital Affiliated to Jiangsu University;Department of Neurology, Third Affiliated Hospital of Navy Military Medical University;Department of Neuroscience, Sheffield Institute for Translational Neuroscience (SITraN), University of Sheffield;Department of Neurosurgery, Shanghai East Hospital, Tongji University School of Medicine;Zhoupu Hospital, Affiliated to Shanghai University of Medicine & Health Sciences;

关键词: Adipose-derived stem cells (ADSCs);    hADSC-derived neuron-like cells (hADSC-NCs);    Middle cerebral artery occlusion (MCAO);    National Institute of Health Stroke Scale (NIHSS);    Rogers scale system;   

DOI  :  10.1186/s13578-022-00774-x

来源: DOAJ

【 摘 要 】

Abstract Background Human adipose-derived stem cells (hADSCs) have been demonstrated to be a promising autologous stem cell source for treating various neuronal diseases. Our study indicated that hADSCs could be induced into neuron-like cells in a stepwise manner that are characterized by the positive expression of MAP2, SYNAPSIN 1/2, NF-200, and vGLUT and electrophysiological activity. We first primed hADSCs into neuron-like cells (hADSC-NCs) and then intracerebrally transplanted them into MCAO reperfusion mice to further explore their in vivo survival, migration, integration, fate commitment and involvement in neural circuit rebuilding. Results The hADSC-NCs survived well and transformed into MAP2-positive, Iba1- or GFAP-negative cells in vivo while maintaining some proliferative ability, indicated by positive Ki67 staining after 4 weeks. hADSC-NCs could migrate to multiple brain regions, including the cortex, hippocampus, striatum, and hypothalamus, and further differentiate into mature neurons, as confirmed by action potential elicitation and postsynaptic currents. With the aid of a cell suicide system, hADSC-NCs were proven to have functionally integrated into the hippocampal memory circuit, where they contributed to spatial learning and memory rescue, as indicated by LTP improvement and subsequent GCV-induced relapse. In addition to infarction size shrinkage and movement improvement, MCAO-reperfused mice showed bidirectional immune modulation, including inhibition of the local proinflammatory factors IL-1α, IL-1β, IL-2, MIP-1β and promotion proinflammatory IP-10, MCP-1, and enhancement of the anti-inflammatory factors IL-15. Conclusion Overall, hADSC-NCs used as an intermediate autologous cell source for treating stroke can rebuild hippocampus neuronal circuits through cell replacement.

【 授权许可】

Unknown