Expression and production of soluble Mycobacterium tuberculosis H37Rv mycosin-3

Biochemistry and Biophysics Reports

Nicolaas Claudius Gey van Pittius¹  Zhuo Fang¹  Wolf-Dieter Schubert² 

[1] DST/NRF Centre of Excellence in Biomedical Tuberculosis Research, US/MRC Centre for Molecular and Cellular Biology, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, University of Stellenbosch, Francie van Zijl Drive, Tygerberg 7505, South Africa;Department of Biochemistry, University of Pretoria, cnr Lynnwood Road and Roper Street, Hatfield, Pretoria 0028, South Africa;

关键词: Mycobacterium tuberculosis;    ESX;    Mycosin-3;    Protein production;    Cloning;   

DOI  :  10.1016/j.bbrep.2016.02.005

来源: DOAJ

【 摘 要 】

Mycobacteria encode five type VII secretion system (T7SS) or ESX for nutrient acquisition and virulence. Mycosins are membrane-anchored components of ESX with serine protease activity but an unidentified substrate range. Establishing the substrate specificity of individual mycosins will help to elucidate individual ESX functions. Mycosin-1 and -3 orthologues from two environmental mycobacterial species, Mycobacterium smegmatis and Mycobacterium thermoresistibile, have been heterologously produced, but mycosins from Mycobacterium tuberculosis (Mtb) remain to be studied. Here we describe the successful production of Mtb mycosin-3 as a first step in investigating its structure and function.

【 授权许可】

Unknown