A dual-channel optogenetic stimulator selectively modulates distinct defensive behaviors

iScience

Nianzhen Du¹  Yan Yin²  Yu Zhao³  Yaning Han³  Lizhu Li³  Dawid Sheng³  Xue Cai³  Liping Wang³  Xin Fu⁴  Pengfei Wei⁵  Lan Yin⁵  Xing Sheng⁶  Changbo Liu⁶  Wenhao Liu⁶ 

[1] Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong 999077, China;University of the Chinese Academy of Sciences, Beijing 100049, China;Department of Electronic Engineering, Beijing National Research Center for Information Science and Technology, Center for Flexible Electronics Technology, and IDG/McGovern Institute for Brain Research, Tsinghua University, Beijing 100084, China;School of Materials Science and Engineering, Hangzhou Innovation Institute, Beihang University, Beijing 100191, China;School of Materials Science and Engineering, Tsinghua University, Beijing 100084, China;Shenzhen Key Lab of Neuropsychiatric Modulation and Collaborative Innovation Center for Brain Science, Guangdong Provincial Key Laboratory of Brain Connectome and Behavior, CAS Center for Excellence in Brain Science and Intelligence Technology, Brain Cognition and Brain Disease Institute (BCBDI), Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen-Hong Kong Institute of Brain Science, Shenzhen Fundamental Research Institutions, Shenzhen 518055, China;

关键词: Optoelectronics;    Neuroscience;    Bioelectronics;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Implantable devices and systems have been emerging as powerful tools for neuroscience research and medical applications. Here we report a wireless, dual-channel optoelectronic system for functional optogenetic interrogation of superior colliculus (SC), a layered structure pertinent to defensive behaviors, in rodents. Specifically, a flexible and injectable probe comprises two thin-film microscale light-emitting diodes (micro-LEDs) at different depths, providing spatially resolved optical illuminations within the tissue. Under remote control, these micro-LEDs interrogate the intermediate layer and the deep layer of the SC (ILSC and DLSC) of the same mice, and deterministically evoke distinct freezing and flight behaviors, respectively. Furthermore, the system allows synchronized optical stimulations in both regions, and we discover that the flight response dominates animals' behaviors in our experiments. In addition, c-Fos immunostaining results further elucidate the functional hierarchy of the SC. These demonstrations provide a viable route to unraveling complex brain structures and functions.

【 授权许可】

Unknown