Proteomics Analysis of Brain Tissue in a Rat Model of Ischemic Stroke in the Acute Phase

Frontiers in Molecular Neuroscience

Yi-Fu Zeng¹  Fei Zheng¹  Yan-Tao Zhou¹  Tao Tang²  Zhao-Yu Yang²  Tao Liu²  Jie-Kun Luo²  Yang Wang² 

[1] College of Electrical and Information Engineering, Hunan University, Changsha, China;Laboratory of Ethnopharmacology, Institute of Integrative Medicine, Xiangya Hospital, Central South University, Changsha, China;

关键词: proteomics;    brain tissue;    acute ischemic stroke;    LC-MS/MS;    omics;   

DOI  :  10.3389/fnmol.2020.00027

来源: DOAJ

【 摘 要 】

Background: Stroke is a leading health issue, with high morbidity and mortality rates worldwide. Of all strokes, approximately 80% of cases are ischemic stroke (IS). However, the underlying mechanisms of the occurrence of acute IS remain poorly understood because of heterogeneous and multiple factors. More potential biomarkers are urgently needed to reveal the deeper pathogenesis of IS.Methods: We identified potential biomarkers in rat brain tissues of IS using an iTRAQ labeling approach coupled with LC-MS/MS. Furthermore, bioinformatrics analyses including GO, KEGG, DAVID, and Cytoscape were used to present proteomic profiles and to explore the disease mechanisms. Additionally, Western blotting for target proteins was conducted for further verification.Results: We identified 4,578 proteins using the iTRAQ-based proteomics method. Of these proteins, 282 differentiated proteins, comprising 73 upregulated and 209 downregulated proteins, were observed. Further bioinformatics analysis suggested that the candidate proteins were mainly involved in energy liberation, intracellular protein transport, and synaptic plasticity regulation during the acute period. KEGG pathway enrichment analysis indicated a series of representative pathological pathways, including energy metabolite, long-term potentiation (LTP), and neurodegenerative disease-related pathways. Moreover, Western blotting confirmed the associated candidate proteins, which refer to oxidative responses and synaptic plasticity.Conclusions: Our findings highlight the identification of candidate protein biomarkers and provide insight into the biological processes involved in acute IS.

【 授权许可】

Unknown