Assessment of Human Mycotoxin Exposure in Hungary by Urinary Biomarker Determination and the Uncertainties of the Exposure Calculation: A Case Study

Foods

Judit Szabó-Fodor¹  Melinda Kovács¹  Brigitta Bóta¹  András Szabó²  Cserne Angeli²  Mária Szeitzné-Szabó²  Tamás Schieszl²  Lucia Gambacorta³  Michele Solfrizzo³ 

[1] Eötvös Lóránd Research Network MTA-KE-SZIE Mycotoxins in the Food Chain Research Group, Kaposvár Campus, Hungarian University of Agricultural and Life Science, Guba S. u. 40, 7400 Kaposvar, Hungary;Institute of Physiology and Nutrition, Kaposvár Campus, Hungarian University of Agricultural and Life Sciences, Guba S. u. 40, 7400 Kaposvar, Hungary;Institute of Sciences of Food Production (ISPA), National Research Council (CNR), 70126 Bari, Italy;

关键词: human exposure;    mycotoxin;    urine;    biomarker;    uncertainty;    Hungary;   

DOI  :  10.3390/foods11010015

来源: DOAJ

【 摘 要 】

Urinary biomarkers of mycotoxin exposure were evaluated in the case of healthy people (n = 41) and coeliac patients (n = 19) by using a multi-biomarker LC-MS/MS immunoaffinity based method capable to analyse biomarkers of nine mycotoxins, i.e., fumonisin B1 (FB1), fumonisin B2 (FB2), deoxynivalenol (DON), zearalenone (ZEN), ochratoxin A (OTA), Aflatoxin B1 (AFB1), T-2 toxin, HT-2 toxin and Nivalenol (NIV). Urinary biomarker concentrations were used to calculate the probable daily intake (PDI) of fumonisin B1, deoxynivalenol, zearalenone and ochratoxin A and compared with their tolerable daily intake (TDI). The human urinary excretion rate values reported in the literature and the 24 h excretion rate measured in piglets were used to estimate and compare the PDI values of the four mycotoxins. The highest mean biomarker concentrations were found for DON (2.30 ng/mL for healthy people and 2.68 ng/mL for coeliac patients). Mean OTA concentration was significantly higher (p < 0.001) in healthy people compared to coeliac patients. PDI calculated with piglets excretion data exceeded the TDI values by a much smaller percentage than when they were calculated from human data, especially for FB₁. The uncertainties arising from the different calculations can be well perceived on the basis of these data.

【 授权许可】

Unknown