Improved autosomal STR typing of degraded femur samples extracted using a custom demineralization buffer and DNA IQ™

Forensic Science International: Synergy

Bea G. Gallardo¹  Gayvelline C. Calacal²  Dame Loveliness T. Apaga³  Maria Corazon A. De Ungria³ 

[1] DNA Analysis Laboratory, Natural Sciences Research Institute, Miranda Hall, University of the Philippines, Diliman, 1101, Quezon City, Philippines;Corresponding author.;DNA Analysis Laboratory, Natural Sciences Research Institute, Miranda Hall, University of the Philippines, Diliman, 1101, Quezon City, Philippines;

关键词: Forensic;    DNA extraction;    Bone;    Demineralization;    Autosomal STR;    Automation;   

DOI  :  

来源: DOAJ

【 摘 要 】

Bone samples are utilized as a source of DNA in disaster victim identification and human remains investigations. However, DNA recovery from bones is time consuming and prone to contamination. A logical approach for postmortem identification is to validate efficient DNA extraction methods requiring less bone material using improved molecular kits with less hands-on time and workflows that facilitate faster turn-around time for processing. In this study, we evaluated DNA yield and amplification efficiency of DNA extracts using a new custom bone demineralization buffer (DMB; Promega) and extracted via manual and automated DNA IQ™ workflows. Including the demineralization step, the bone protocol can be completed in ∼4 h and even less with minimal sample handling when automated. Overall, a rapid and simple DNA extraction with improved allele recovery was validated using degraded bone samples exposed to tropical environments and post-disaster recovery as well as adverse conditions of embalming prior to internment.

【 授权许可】

Unknown