Epidemiology and Characterization of CTX-M-55-Type Extended-Spectrum β-Lactamase-Producing Salmonella enterica Serovar Enteritidis Isolated from Patients in Shanghai, China

Microorganisms

Jia Chen¹  Baowei Yang²  Qinya Niu²  Huanjing Sheng²  Bin Liu²  Chenyang Cao²  Shenghui Cui³  Xuebin Xu⁴ 

[1] College of Chemical Technology, Shijiazhuang University, Shijiazhuang 050035, China;College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China;National Institutes for Food and Drug Control, Beijing 100050, China;Shanghai Municipal Center for Disease Control & Prevention, Shanghai 200336, China;

关键词: Salmonella Enteritidis;    human patients;    extended-spectrum β-lactamases;    bla_CTX-M-55;    pulsed-field gel electrophoresis;   

DOI  :  10.3390/microorganisms9020260

来源: DOAJ

【 摘 要 】

The emergence of extended-spectrum β-lactamase-producing Salmonella enterica serovar Enteritidis (ESBL-SE) in humans and foods has gained global attention. In particular, CTX-M-type ESBL-SE are increasingly being detected from various sample types. The aim of this study was to comprehensively analyze the epidemiology and characteristics of bla_CTX-M-55-carrying ESBL-SE isolates of clinical origin in Shanghai, China. A total of 292 S. Enteritidis isolates were recovered from the feces and blood of outpatients and inpatients between 2006 and 2014. Overall, there was a high frequency of cefotaxime-resistant isolates (97.3%), which was significantly higher (p < 0.01) than that of isolates resistant to the other tested antibiotics. All S. Enteritidis isolates exhibited resistance to ≥1 antibiotic, and 98.0% were multidrug resistant. A total of 233 isolates were identified as ESBL-SE, 166 of which were CTX-M type. Six subtypes of CTX-M-encoding genes were detected, among which bla_CTX-M-55 (91.6%, 152/166) was the most prevalent genotype. There was high genetic similarity among bla_CTX-M-55-positive ESBL-SE. The bla_CTX-M-55 gene in the ESBL-SE donor strains could be easily transferred into Enterobacteriaceae recipient strains. This study highlights that CTX-M-55 should be considered an important surveillance target in Shanghai, China. Cephalosporins, especially cefotaxime, must be used with caution in empirical treatment for Salmonella infections.

【 授权许可】

Unknown