Lipidomic and Transcriptomic Basis of Lysosomal Dysfunction in Progranulin Deficiency

Cell Reports

Joachim Herz¹  Carlos Rodriguez-Navas¹  Catherine R. Wasser¹  Rachel J. Tesla¹  Kyoung Shin Yoo¹  Gang Yu¹  Janine Prange-Kiel¹  Bret M. Evers¹  Florian Plattner¹  Jeffrey McDonald²  Basar Cenik²  Thomas A. Ravenscroft³  Rosa Rademakers³  Charles L. White, III⁴ 

[1] Center for Translational Neurodegeneration Research, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA;Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA;Department of Neuroscience, Mayo Clinic, Jacksonville, FL 32224, USA;Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA;

关键词: neurodegeneration;    Alzheimer’s disease;    frontotemporal lobar degeneration;    lipids;    lipidomics;    transcriptomics;    lysosome;    lysosomal storage disorders;    neuronal ceroid lipofuscinoses;   

DOI  :  10.1016/j.celrep.2017.08.056

来源: DOAJ

【 摘 要 】

Defective lysosomal function defines many neurodegenerative diseases, such as neuronal ceroid lipofuscinoses (NCL) and Niemann-Pick type C (NPC), and is implicated in Alzheimer’s disease (AD) and frontotemporal lobar degeneration (FTLD-TDP) with progranulin (PGRN) deficiency. Here, we show that PGRN is involved in lysosomal homeostasis and lipid metabolism. PGRN deficiency alters lysosome abundance and morphology in mouse neurons. Using an unbiased lipidomic approach, we found that brain lipid composition in humans and mice with PGRN deficiency shows disease-specific differences that distinguish them from normal and other pathologic groups. PGRN loss leads to an accumulation of polyunsaturated triacylglycerides, as well as a reduction of diacylglycerides and phosphatidylserines in fibroblast and enriched lysosome lipidomes. Transcriptomic analysis of PGRN-deficient mouse brains revealed distinct expression patterns of lysosomal, immune-related, and lipid metabolic genes. These findings have implications for the pathogenesis of FTLD-TDP due to PGRN deficiency and suggest lysosomal dysfunction as an underlying mechanism.

【 授权许可】

Unknown