Data-deposition protocols for correlative soft X-ray tomography and super-resolution structured illumination microscopy applications

STAR Protocols

Ardan Patwardhan¹  Maria Harkiolaki²  Mohamed A. Koronfel²  Tom Fish²  Gerard J. Kleywegt³  Ugis Sarkans³  Andrii Iudin³  Ian Dobbie⁴ 

[1] Corresponding author;Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 0DW, UK;European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge CB10 1SD, UK;Micron, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, UK;

关键词: Bioinformatics;    Cell Biology;    Microscopy;    Structural Biology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: This protocol illustrates the steps necessary to deposit correlated 3D cryo-imaging data from cryo-structured illumination microscopy and cryo-soft X-ray tomography with the BioStudies and EMPIAR deposition databases of the European Bioinformatics Institute. There is currently a real need for a robust method of data deposition to ensure unhindered access to and independent validation of correlative light and X-ray microscopy data to allow use in further comparative studies, educational activities, and data mining.For complete details on the use and execution of this protocol, please refer to Kounatidis et al. (2020).

【 授权许可】

Unknown