Epigenetic Regulation of Aldosterone Synthase Gene by Sodium and Angiotensin II

Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Masashi Demura¹  Yoshiyu Takeda²  Mitsuhiro Kometani²  Daisuke Aono²  Yoshimichi Takeda²  Shigehiro Karashima²  Takashi Yoneda²  Fen Wang²  Masakazu Yamagishi²  Atsushi Hashimoto²  Makiko Meguro‐Horike³  Shin‐ichi Horike³ 

[1] Department of Hygiene, Kanazawa University School of Medicine, Kanazawa, Japan;Department of Internal Medicine, Kanazawa University School of Medicine, Kanazawa, Japan;Division of Functional Genomics, Kanazawa University Advanced Science Research Center, Kanazawa, Japan;

关键词: aldosterone;    angiotensin II;    CYP11B2;    epigenetics;    sodium;   

DOI  :  10.1161/JAHA.117.008281

来源: DOAJ

【 摘 要 】

BackgroundDNA methylation is believed to be maintained in adult somatic cells. Recent findings, however, suggest that all methylation patterns are not stable. We demonstrate that stimulatory signals can change the DNA methylation status around transcription factor binding sites and a transcription start site and activate expression of the aldosterone synthase gene (CYP11B2). Methods and ResultsDNA methylation of CYP11B2 was analyzed in aldosterone‐producing adenomas, nonfunctioning adrenal adenomas, and adrenal glands and compared with the gene expression levels. CpG dinucleotides in the CYP11B2 promoter were found to be hypormethylated in tissues with high expression, but not in those with low expression, of CYP11B2. Methylation of the CYP11B2 promoter fused to a reporter gene decreased transcriptional activity. Methylation of recognition sequences of transcription factors, including CREB1, NGFIB (NR4A1), and NURR1 (NR4A2) diminished their DNA‐binding activity. A methylated‐CpG‐binding protein MECP2 interacted directly with the methylated CYP11B2 promoter. In rats, low salt intake led to upregulation of CYP11B2 expression and DNA hypomethylation in the adrenal gland. Treatment with angiotensin II type 1 receptor antagonist decreased CYP11B2 expression and led to DNA hypermethylation. ConclusionsDNA demethylation may switch the phenotype of CYP11B2 expression from an inactive to an active state and regulate aldosterone biosynthesis.

【 授权许可】

Unknown